CRYSTALLOGRAPHIC STUDIES OF INTERLEUKIN 5 RECEPTOR

我们已经将β亚基的细胞外结构域 白细胞介素-5受体。 这是一种重要的细胞因子受体 与哮喘的发病机制有关， 寄生虫感染 晶体衍射很弱， 在旋转阳极上收集完整的数据集需要10天 源头 晶体的有效分辨率受到晶体的光学性质的限制。 数据采集系统的信噪比为-3.5A， 旋转阳极和同步加速器上的-2.6A。 为了方便 通过衍生化，已经产生了许多半胱氨酸突变体。 一些变异晶体只能长到很小的尺寸。 这些不 当使用旋转阳极源时， 已经显示 这些晶体在14 BM-C上产生有用的衍射。

We have crystallised the extracellular domain of the beta subunit of the interleukin-5 receptor. This is an important cytokine receptor that has been implicated in the pathogensis of asthma and certain parasite infections. The crystals are weakly diffracting and it can take up to 10 days to collect a complete dataset on a rotating anode source. The effective resolution of the crystals is limited by the signal to noise ratio of the data collection system, being -3.5A on a rotaing anode and -2.6A on a synchrotron. In order to facilitate derivativisation, a number of cysteine mutants have been produced. Some of the mutant crystals grow only to a small size. These do not diffract when a rotating anode source is used. It has been shown that these crystals produce usable diffraction on 14 BM-C.