CRYSTALLOGRAPHIC STUDIES OF INTERLEUKIN 5 RECEPTOR

我们已经将β亚基的细胞外结构域 白细胞介素-5受体。 这是一种重要的细胞因子受体 它与哮喘的发病机制有关， 寄生虫感染 为了便于衍生化， 已经产生了许多半胱氨酸突变体。 一些变种人 晶体只能生长到很小的尺寸。 利用同步辐射， 应该能够（a）提高我们已知数据的分辨率 导数，（B）使来自汞原子的异常信号最大化 (c)在结冰成为问题之前迅速收集数据，以及（d） 从其他潜在衍生突变体的晶体中收集数据 它们不会长得大到足以对我们的常规源起反作用。 使用BioCARS Station 14-BM-D。

We have crystallised the extracellular domain of the beta subunit of the interleukin-5 receptor. This is an important cytokine receptor that has been implicated in the pathogensis of Asthma and certain parasite infections. In order to facilitate derivativisation, a number of cysteine mutants have been produced. Some of the mutant crystals grow only to a small size. By using synchrotron radiation we should be able to (a) increase the resolution of data from our known derivative, (b) maximise the anomalous signal from the mercury atoms (c) collect data rapidly before icing becomes a problem, and (d) collect data from crystals of other potentially derivative mutants which do not grow large enough to diffract on our conventional source. BioCARS Station 14-BM-D was used.