Three-dimensional cryo correlative light and electron microscopy facility

三维冷冻关联光学和电子显微镜设施

• 批准号: LE180100202
• 负责人: A/Prof Georg Ramm
• 金额: $ 17.01万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2018
• 资助国家: 澳大利亚
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE180100202
• 关键词: Three; dimensional; cryo; correlative; light

This project aims to establish a three-dimensional (3D) cryo-correlative light and electron microscopy facility. The facility will integrate light microscopy with high resolution cryo-electron tomography and 3D slice-and-view focused ion beam scanning electron microscopy. The open access facility should create new capabilities for Australian researchers to tag biological events and structures with fluorescence markers and image them using the currently highest resolution 3D imaging techniques for biological matter. The facility expects to reveal fundamental insights into cell and structural biology, and help drive innovation in agriculture, pharmaceutics, and biomaterials.

该项目旨在建立一个三维（3D）冷冻相关的光学和电子显微镜设备。该设施将集成光学显微镜与高分辨率冷冻电子断层扫描和三维切片和视图聚焦离子束扫描电子显微镜。开放获取设施将为澳大利亚研究人员创造新的能力，用荧光标记标记生物事件和结构，并使用目前最高分辨率的生物物质3D成像技术对其进行成像。该设施预计将揭示细胞和结构生物学的基本见解，并帮助推动农业，制药和生物材料的创新。