ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
批准号:
3073497
负责人:
MICHAEL S. FREEMARK
金额:
$6.6万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-05-01 至 1995-04-30
关键词:
complementary DNA densitometry embryo /fetus embryo /fetus cell /tissue gel electrophoresis gestational age hormone binding protein hormone receptor hormone regulation /control mechanism liver liver cells mammalian embryology messenger RNA molecular cloning nucleic acid probes placental hormones pregnancy disorder prenatal growth disorder receptor expression sheep somatomammotropin statistics /biometry tissue /cell culture
中文摘要
胎盘催乳素(PL)具有直接的代谢和促生长作用,
胎儿组织,这表明激素在控制
胎儿生长 PL在胎儿中的生物学作用是有药物作用的
通过与独特的PL受体结合。 整体
这项建议的目的是研究的生理和调节,
PL受体在细胞和分子水平。 基因的mRNA
绵羊PL受体将使用分离自
胎羊肝cDNA文库 孕母胎盘组织中PL受体mRNA水平
在怀孕的各个阶段检查胎羊组织,
确定开发过程中PL绑定的变化是否导致
PL受体mRNA表达的变化。 调控
PL受体mRNA的营养和激素因素将在
胎羊和母体绵羊肝脏以及
体外 这些研究应该会产生新的信息,
Pl受体在分子水平上的作用,可能有助于阐明
各种激素和营养物质调节表达的机制
妊娠母羊和胎儿羔羊的PL受体。 因为PL有
促进胎儿组织生长的作用,这些研究还应
提供了控制胎儿生长的机制,在正常和
病理性妊娠 为了进行分子研究
PL受体的生物学,我已经寻求专家的帮助,
在分子内分泌学方面有丰富的经验。 此外,作为一个主要
RCDA的组成部分,我计划15个月的休假,以获得培训,
分子生物学的理论和技术。 RCDA将提供
有时间和机会探索这一新的研究领域,从而
加强我作为胎儿领域独立调查员的职业生涯
内分泌学
英文摘要
Placental lactogen (PL) has direct metabolic and somatotrophic effects in
fetal tissues, suggesting that the hormone plays a role in the control of
fetal growth. The biological actions of PL in the fetus are medicated
through binding to a distinct and unique PL receptor. The overall
objective of this proposal is to examine the physiology and regulation of
the PL receptor at the cellular and molecular levels. The mRNA for the
sheep PL receptor will be characterized using a cDNA probe isolated from a
fetal sheep liver cDNA library. The levels of PL receptor mRNA in maternal
and fetal sheep tissues will be examined at various stages of pregnancy to
determine whether changes in the binding of PL during development result
from changes in the expression of the PL receptor mRNA. The regulation of
the PL receptor mRNA by nutritional and hormonal factors will be studied in
fetal and maternal sheep liver in vivo and in ovine fetal hepatocytes in
vitro. These studies should yield new information regarding the physiology
of the Pl receptor at the molecular level and may help to clarify the
mechanisms by which various hormones and nutrients regulate the expression
of the PL receptor in the pregnant ewe and fetal lamb. Because PL has
growth-promoting actions in fetal tissues, these studies should also
provide insight into the mechanisms controlling fetal growth in normal and
pathological pregnancies. In order to conduct studies of the molecular
biology of the PL receptor, I have solicited the assistance of experts with
extensive experience in molecular endocrinology. In addition, as a major
component of the RCDA, I plan a 15 month sabbatical to obtain training in
the theory and techniques of molecular biology. An RCDA would provide the
time and opportunity to explore this new area of research and would thereby
enhance my career as an independent investigator in the field of fetal
endocrinology.
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会议论文
METFORMIN IN OBESE ADOLESCENTS PREDISPOSED TO TYPE II DIABETES
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批准号:6565328
-
项目类别:
-
资助金额:$11.7万
-
财政年份:2001
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
METFORMIN IN OBESE ADOLESCENTS PREDISPOSED TO TYPE II DIABETES
-
批准号:6415269
-
项目类别:
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资助金额:$29.31万
-
财政年份:2000
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负责人:MICHAEL S. FREEMARK
-
依托单位:
METFORMIN IN OBESE ADOLESCENTS PREDISPOSED TO TYPE II DIABETES
-
批准号:6503068
-
项目类别:
-
资助金额:$11.7万
-
财政年份:2000
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
METFORMIN IN OBESE ADOLESCENTS PREDISPOSED TO TYPE II DIABETES
-
批准号:6463031
-
项目类别:
-
资助金额:$11.7万
-
财政年份:2000
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
METFORMIN IN OBESE ADOLESCENTS PREDISPOSED TO TYPE II DIABETES
-
批准号:6112763
-
项目类别:
-
资助金额:$4.09万
-
财政年份:1998
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
DIABETES PREVENTION TRIAL--TYPE 1
-
批准号:6273998
-
项目类别:
-
资助金额:$2.88万
-
财政年份:1997
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
METFORMIN IN OBESE ADOLESCENTS PREDISPOSED TO TYPE II DIABETES
-
批准号:6273997
-
项目类别:
-
资助金额:$2.88万
-
财政年份:1997
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
-
批准号:2292449
-
项目类别:
-
资助金额:$1.63万
-
财政年份:1994
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
-
批准号:3073494
-
项目类别:
-
资助金额:$6.61万
-
财政年份:1990
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
-
批准号:3073495
-
项目类别:
-
资助金额:$6.6万
-
财政年份:1990
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
-
批准号:3073496
-
项目类别:
-
资助金额:$6.6万
-
财政年份:1990
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
-
批准号:2194384
-
项目类别:
-
资助金额:$6.6万
-
财政年份:1990
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
PLACENTAL LACTOGEN AND FETAL DEVELOPMENT
-
批准号:2883124
-
项目类别:
-
资助金额:$24.0万
-
财政年份:1988
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
ROLE OF PLACENTAL LATOGEN IN FETAL DEVELOPMENT
-
批准号:3324657
-
项目类别:
-
资助金额:$13.06万
-
财政年份:1988
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
THE ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
-
批准号:6758018
-
项目类别:
-
资助金额:$34.65万
-
财政年份:1988
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
PLACENTAL LACTOGEN AND FETAL DEVELOPMENT
-
批准号:2463096
-
项目类别:
-
资助金额:$23.51万
-
财政年份:1988
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
THE ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
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批准号:3324660
-
项目类别:
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资助金额:$8.82万
-
财政年份:1988
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
PLACENTAL LACTOGEN AND FETAL DEVELOPMENT
-
批准号:6164891
-
项目类别:
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资助金额:$24.72万
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财政年份:1988
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负责人:MICHAEL S. FREEMARK
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依托单位:
THE ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
-
批准号:2199085
-
项目类别:
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资助金额:$9.28万
-
财政年份:1988
-
负责人:MICHAEL S. FREEMARK
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依托单位:
THE ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
-
批准号:6687646
-
项目类别:
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资助金额:$32.95万
-
财政年份:1988
-
负责人:MICHAEL S. FREEMARK
-
依托单位:
国内基金
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批准号:81200899
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资助金额:23.0万元
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批准年份:2012
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负责人:李琦
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依托单位: