THE MAJOR MYELIN GLYCOPROTEIN
THE MAJOR MYELIN GLYCOPROTEIN
批准号:
3078282
负责人:
JAMES SALZER
金额:
$4.4万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-07-01 至 1989-06-30
关键词:
complementary DNA electron microscopy gel electrophoresis gene expression genetic library genetic manipulation genetic regulation genetic transcription membrane structure messenger RNA molecular cloning myelin myelination myelinopathy neurophysiology nucleic acid sequence structural genes tissue /cell culture
中文摘要
该项目的长期目标是描述早期事件的特征
并了解髓鞘形成的过程。
特定髓鞘蛋白的基因表达受到调控。对这件事
最后,主要外周髓鞘糖蛋白的基因Po和
髓鞘相关糖蛋白(MAG),将被研究。Po是一个积分
膜蛋白,是主要的外周髓鞘蛋白;它很可能
在外围设备的结构完整性中发挥重要作用
髓鞘。MAG在髓鞘内有一个定位
暗示了在调节神经胶质细胞-神经元相互作用中的作用;它也是
优先消失在多发性硬化症的急性斑块中,它是
副蛋白抗原靶向进一步诱导周围神经病变
强调其潜在的重要性。MAG和Po的cDNA克隆将是
被共同的战略所孤立。抗纯化MAG和Po Will的抗体
用于在体外翻译实验中鉴定信使核糖核酸组分
从富含序列的小鼠周围神经中提取
MAG和PO的编码。这些组分将被用作探针来分离
来自小鼠周围神经文库的各自的cDNA(将被
准备好了)。蛋白质的一级结构将来自于
MAG和Po cDNA的核苷酸序列也将用于
从基因组文库中分离Po和MAG基因。《解释》
基因的结构和外显子内关系的评估
可能有助于确定蛋白质的可能功能结构域。
Po和PLP的电子显微镜异源双链分析可能揭示共同的
反映共同进化起源的序列。它的缺陷就是
外周髓鞘脱髓鞘障碍小鼠突变颤抖,将通过
测定MAG、Po和MBPS的mRNA水平。长期目标包括
外周血髓鞘蛋白基因表达调控的研究
髓鞘组织培养体系。
英文摘要
Long term objectives of this project are to characterize early events of
myelinogenesis and to gain an understanding of the process by which the
expression of genes for specific myelin proteins is regulated. To this
end, the genes for the major peripheral myelin glycoproteins, Po and the
myelin associated glycoprotein (MAG), will be studied. Po is an integral
membrane protein and is the major peripheral myelin protein; it is likely
to play and important role in the structural integrity of the peripheral
myelin sheath. MAG has a localization within the myelin sheath that
suggests a role in mediating glial-neuronal interactions; it is also
preferentially lost in acute plaques of multiple sclerosis and it is the
antigenic target of paraprotein induced peripheral neuropathies further
underscoring its potential importance. cDNA clones for MAG and Po will be
isolated by a common strategy. Antibodies against purified MAG and Po will
be used to identify, in vitro translation experiments, mRNA fractions
extracted from mouse peripheral nerves, which are enriched in sequences
coding for MAG and Po. These fractions will be used as probes to isolate
the respective cDNAs from a mouse peripheral nerve library (to be
prepared). The primary structure of the proteins will be derived from the
nucleotide sequence of the MAG and Po cDNAs which will also be used to
isolate the genes for Po and MAG from a genomic library. The elucidation
of the structure of the genes and an evaluation of intra-exon relationships
may be useful in identifying possible functional domains of the proteins.
Electron microscopic heteroduplex analysis of Po and PLP may reveal common
sequences reflecting a common evolutionary origin. The defect in the
peripheral dysmyelinating mouse mutatn Trembler, will be studied by
determining mRNA levels of MAG, Po and the MBPs. Long range goals include
studying the regulation of myelin protein gene expression in a peripheral
myelin tissue culture system.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Impact of Schwann Cell Pathology on Axon Structure and Function
-
批准号:10568051
-
项目类别:
-
资助金额:$57.21万
-
财政年份:2022
-
负责人:JAMES SALZER
-
依托单位:
Role and Regulation of Neural Stem Cells in Remyelination
-
批准号:10412936
-
项目类别:
-
资助金额:$42.47万
-
财政年份:2018
-
负责人:JAMES SALZER
-
依托单位:
Role and Regulation of Neural Stem Cells in Remyelination
-
批准号:10155591
-
项目类别:
-
资助金额:$42.47万
-
财政年份:2018
-
负责人:JAMES SALZER
-
依托单位:
Mechanisms of Node of Ranvier Assembly
-
批准号:8675608
-
项目类别:
-
资助金额:$35.75万
-
财政年份:2013
-
负责人:JAMES SALZER
-
依托单位:
Regulation of Schwann cell enshealthment and myelination by type III Neuregulin 1
-
批准号:8675621
-
项目类别:
-
资助金额:$35.65万
-
财政年份:2013
-
负责人:JAMES SALZER
-
依托单位:
2012 Myelin Gordon Research Conference & Gordon Research Seminar
-
批准号:8317793
-
项目类别:
-
资助金额:$2.0万
-
财政年份:2012
-
负责人:JAMES SALZER
-
依托单位:
Assembly of the Node of Ranvier
-
批准号:7263674
-
项目类别:
-
资助金额:$36.97万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Assembly of the Node of Ranvier
-
批准号:8049575
-
项目类别:
-
资助金额:$36.34万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Mechanisms of Node of Ranvier Assembly
-
批准号:10212457
-
项目类别:
-
资助金额:$46.98万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Assembly of the Node of Ranvier
-
批准号:7586695
-
项目类别:
-
资助金额:$37.08万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Assembly of the Node of Ranvier
-
批准号:7363673
-
项目类别:
-
资助金额:$37.03万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Node of Ranvier Assembly: Role of Axo-Glial Interactions
-
批准号:6875654
-
项目类别:
-
资助金额:$36.12万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Assembly of the Node of Ranvier
-
批准号:7794972
-
项目类别:
-
资助金额:$36.71万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Node of Ranvier Assembly: Role of Axo-Glial Interactions
-
批准号:6740167
-
项目类别:
-
资助金额:$36.12万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Mechanisms of Node of Ranvier Assembly
-
批准号:8439196
-
项目类别:
-
资助金额:$37.08万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Assembly of the Node of Ranvier
-
批准号:7873109
-
项目类别:
-
资助金额:$9.67万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Mechanisms of Node of Ranvier Assembly
-
批准号:10052763
-
项目类别:
-
资助金额:$46.98万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Mechanisms of Node of Ranvier Assembly
-
批准号:10655367
-
项目类别:
-
资助金额:$46.98万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Mechanisms of Node of Ranvier Assembly
-
批准号:8542904
-
项目类别:
-
资助金额:$35.78万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
Node of Ranvier Assembly: Role of Axo-Glial Interactions
-
批准号:6466178
-
项目类别:
-
资助金额:$35.07万
-
财政年份:2002
-
负责人:JAMES SALZER
-
依托单位:
海外基金