BASEMENT MEMBRANE ZONE IN VOCAL INJURY

声带损伤中的基底膜区

• 批准号: 3080284
• 负责人: STEVEN D GRAY
• 金额: $ 9.34万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1994-05-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3080284
• 关键词: basement membrane; dogs; edema; histology; human tissue; immunoelectron microscopy; immunofluorescence technique; injury; larynx disorder; membrane structure; morphology; skin; vocal cords

This project investigates the epidermal-lamina poropria junction, knwon as the basement membrane zone (BMZ). The basic questions are, "What holds the epidermis to the fibrous structures of the lamina propria?" and "Why is BMZ pathology so frequent in benign laryngeal disease?" Histology of human vocal nodules and Reinke's edema suggests the BMZ plays an important role in vocal injury. The BMZ in skin is composed of attaching stuctures, such as hemidesmosomes and anchoring fibrils which help secure the lamina propria to the epidermis. The BMZ in skin also contains many proteins which help hold or "glue" the BMZ intact. Through electorn microscopy, morphometric studies will be performed to determine the population density of the attaching BMZ structures in normal human and canine vocal cords. Since dermatiological studies have shown variation in population densities of attaching structures across individuals, the project will determine normal ranges for attaching BMZ structures. Through immunofluorescent, and immunoelectron microscopic techniques, the proteins of the BMZ will be characterized in normal human cords. After normative data has been obtained, it will be used to help determine abnormalities of the BMZ in injured cords. It is suspected that vocal nodules are the result of injury to BMZZ structures. Vocal nodules, polyps or Reinke's edema specimens will be examined to determine if a) certain individuals may inherently have a low number of attaching structures or proteins leading to a lower threshold for phonatory injury and b) chronic injury to these structures lead to biological changes in the BMZ. Additionally, acute canine models of phonatory injury will be examined for BMZ injury. The investigation will identify both the architecture (using electron microscopy) and the biological composition (using immunoflourescence techniques) of the BMZ. Pathogenesis of vocal injury will be studied witb interest towards possible anatomical factors which may impose some vocal cords to injury. It is the long range plan to acquire greater skills in state of the art electron microscopic techniques and immunofluorescent techniques to investigate laryngeal anatomic and biological components. With these skills, benign laryngeal disease causing phonatory dysfunction and therapy will be studied.

本项目研究表皮-多孔层连接， 称为基底膜区（BMZ）。 根本问题 是什么把表皮和皮肤的纤维结构 固有层和“为什么BMZ病理在良性肿瘤中如此频繁 咽喉疾病？“人类声带小结的组织学和Reinke's 提示BMZ在声带损伤中起重要作用。 皮肤中的BMZ由附着结构组成，如 半桥粒和锚定纤维有助于固定椎板 固有到表皮。 皮肤中的BMZ也含有许多 帮助保持或“粘合”BMZ完整的蛋白质。 通过电子显微镜，将进行形态学研究 以确定附着的BMZ结构的群体密度 在正常人和狗的声带中。 由于皮肤病学 研究表明， 跨个人的结构，该项目将确定正常 用于连接BMZ结构的范围。 通过免疫荧光， 和免疫电镜技术，BMZ的蛋白质 将以正常人的声带为特征。 标准数据后 它将被用来帮助确定异常 BMZ在受伤的脊髓中的作用 声带小结可能是BMZZ损伤的结果 结构. 声带小结、息肉或Reinke水肿标本将 检查以确定a）某些个人是否可能固有地 具有少量的附着结构或蛋白质， 发声损伤的较低阈值和B）慢性损伤， 这些结构导致BMZ中的生物变化。 此外，还将建立发音损伤的急性犬模型。 检查BMZ损伤。 调查将确定体系结构（使用 电子显微镜）和生物组成（使用 免疫荧光技术）。 声带病理 将对损伤进行研究， 这些因素可能会对声带造成损伤。 这是一个长期计划，以获得更大的技能， 电子显微镜技术和免疫荧光技术 喉的解剖学和生物学研究技术 件. 有了这些技能，良性喉疾病引起的 将研究发声功能障碍和治疗。