MOLECULAR BIOLOGY OF MEMORY

这项申请的长期目标将是进一步 学习记忆分子基础的理解与应用 这一知识有助于疾病的研究，如阿尔茨海默病。它 人们普遍认为长时记忆的获得是一个过程 这取决于蛋白质的合成。因此，有人推测 这个过程与一个或多个基因的表达有关，这些基因可能 参与细胞内的长期适应性修改。研究 无脊椎动物行为的研究揭示了学习的简单形式是 以分子和离子事件为特征的，导致 突触功能。这种变化也会发生在长时程增强中 (LTP)被认为是一种神经可塑性和长时间损伤的模型。 哺乳动物神经系统中的术语记忆处理。此次收购 与长期记忆的过程有着惊人的相似之处 在细胞生长和分化过程中。因此，此应用程序将 重点定义LTP背后的分子事件 原癌基因c-fos的表达水平。原癌基因，如 C-fos，被认为在细胞生长和 差异化。因此，有可能增强的神经元 与LTP相关的活性与c-for诱导有关。我们将使用 C-fos基因作为探针，进行Northern杂交和原位杂交 评估c-myc表达水平和定位信号的研究 LTP过程中大鼠和兔海马区FOS信息传递。 将采用免疫组织化学技术来定位Fos蛋白。 通过这种方式，一个或多个在记忆机制和 可以确定LTP的解剖底物。C-半胱氨酸水平的变化 Fos的表达与年龄的关系也将被研究。希望这些都是 研究将有助于进一步了解分子基础。 正常和不正常的认知功能。

The long term objectives of this application will be to further the understanding of the molecular basis of learning and memory and to apply this knowledge to studies of diseases, such as Alzheimer's Disease. It is widely accepted that the acquisition of long-term memory is a process which depends upon protein synthesis. Hence, it has been postulated that this process is linked to the expression of one or more genes which may be involved in long-term adaptive modifications within the cell. Studies of invertebrate behavior have disclosed that simple forms of learning are characterized by molecular and ionic events with result in changes in synaptic function. Such changes also occur with long-term potentiation (LTP) which has been proposed as a model of neural plasticity and long- term memory processing in the mammalian nervous system. The acquisition of long-term memory bears a striking resemblance to processes which occur during cell growth and differentiation. Therefore, this application will focus on defining the molecular events underlying LTP by examining the level of expression of a proto-oncogene, c-fos. Protooncogenes, such as c-fos, are thought to play a regulatory role in cell growth and differentiation. Hence, it is possible that the heightened neuronal activity associated with LTP is related to c-for induction. We will use c-fos cDNA as a probe and perform Northern blot and in situ hybridization studies to evaluate the level of expression and localize the signal of c- fos message in the rat and rabbit hippocampus during LTP. Immunohistochemical techniques will be conducted to localize Fos protein. In this way, a gene or genes which are important in memory mechanisms and an anatomic substrate of LTP may be identified. Changes in levels of c- fos expression with age will also be studied. It is hoped that these studies will contribute to further understanding of the molecular basis of normal and abnormal cognitive function.