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GENE EXPRESSION DURING THE DUCT CELL DIFFERENTIATION IN SALIVARY GLANDS

唾液腺导管细胞分化过程中的基因表达

基本信息

批准号：
17590154
负责人：
ISEKI Shoichi
金额：
$ 2.3万
依托单位：
KANAZAWA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

1)A novel protein expressed in rat sublingual gland : We cloned a novel rat gene expressed primarily in the sublingual gland and named the predicted 503 amino-acid protein SLAMP (sublingual acinar membrane protein). SLAMP had 63% homology with human ERGIC-53-like protein, a member of the family of animal L-type lectins. SLAMP mRNA and protein were expressed predominantly in the mucous acinar cells of sublingual gland. Ultrastructurally, SLAMP was localized to the ER-Golgi intermediate compartment (ERGIC), suggesting that SLAMP functions in the early secretory pathway of glycoproteins. 2)A new method of producing rat polyclonal antibodies with shorter time and lower cost: Producing antibodies usually takes a long time. We developed a new method of producing polyclonal antibodies in less than a month, based on preparation of the recombinant oligopeptides as antigen followed by immunization of rats. Using this method, we produced antisera against ERGIC-53 and confirmed its localization in the ERGIC. 3)A novel submandibular androgen-repressed protein of the mouse: We characterized a cDNA clone derived from the female mouse submandibular gland (SMG). The protein product of this clone had a 165-amino acid protein with a putative signal sequence and was named submandibular androgen-repressed protein (SMARP). In the mouse SMARP mRNA was expressed only in the SMG and exorbital lacrimal gland (LG). The level of SMARP mRNA was 36 times higher in female than male SMG, whereas it was 28 times higher in male than female LG. Furthermore, it was reduced in SMG but increased in LG after administration of testosterone to females or castrated males. SMARP mRNA and protein were predominantly localized in acinar cells in both SMG and lacrimal gland. These results suggested that SMARP is a secretory protein whose expression is regulated by androgens negatively in the SMG and positively in the LG.

1)在大鼠舌下腺中表达的新蛋白：我们克隆了一个主要在大鼠舌下腺中表达的新基因，并将预测的503氨基酸蛋白命名为SLAMP （subblatinar membrane protein，舌下腺泡膜蛋白）。SLAMP与人类ergic -53样蛋白（动物l型凝集素家族成员）具有63%的同源性。SLAMP mRNA和蛋白主要在舌下腺粘液腺泡细胞中表达。在超微结构上，SLAMP定位于er -高尔基间室（ERGIC），提示SLAMP在糖蛋白的早期分泌途径中起作用。2)一种生产大鼠多克隆抗体的新方法，时间短，成本低：生产抗体通常需要很长时间。我们开发了一种新的方法，在不到一个月的时间内制备重组寡肽作为抗原，然后免疫大鼠。用这种方法制备了ERGIC-53的抗血清，并证实了其在ERGIC中的定位。3)一种新的小鼠下颌骨雄激素抑制蛋白：我们鉴定了来自雌性小鼠下颌骨腺（SMG）的cDNA克隆。该蛋白产物含有一个165个氨基酸的蛋白，并具有一个推测的信号序列，被命名为下颌下雄激素抑制蛋白（sub颌骨雄激素抑制蛋白，SMARP）。在小鼠中，SMARP mRNA仅在眶外泪腺（LG）和眶外泪腺中表达。女性SMG的SMARP mRNA水平比男性高36倍，而男性LG的SMARP mRNA水平比女性LG高28倍。此外，在雌性或阉割雄性给予睾酮后，SMG减少，LG增加。SMARP mRNA和蛋白主要定位于SMG和泪腺的腺泡细胞。这些结果表明，SMARP是一种分泌蛋白，其表达受雄激素的调节，在SMG中呈负向，在LG中呈正向。