INVESTIGATION OF A TESTIS PARACRINE FACTOR, P-MOD-S

睾丸旁分泌因子 P-MOD-S 的研究

• 批准号: 3087059
• 负责人: JOHN N. NORTON
• 金额: $ 5.31万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3087059
• 关键词: RNA; Sertoli cells; biological signal transduction; cell cell interaction; cyclic AMP; electrophoresis; gene expression; hormone regulation /control mechanism; ion exchange chromatography; laboratory rat; nucleic acid hybridization; nucleic acid probes; spermatogenesis; testis; transferrin

Testicular somatic cells plays an integral role in the maintenance and control of spermatogenesis. Analysis of somatic cell-cell interactions within the testis has demonstrated that testicular peritubular cells secrete P-Mod-S, a locally derived, nonmitogenic paracrine factor. P-Mod-S the functions of Sertoli cells maintains germinal cell development. P-Mod-S has been shown to stimulate Sertoli cells to a greater degree than any single regulatory agent known to influence the cell. Therefore, peritubular cell-Sertoli cell interactions, mediated via P-Mod-S, is postulated to play a critical role in the maintenance and control of testicular function. The goals of this research proposal are:1) determine the mechanism of action of P-Mod-S on the Sertoli cell;2) determine the effects of P-Mod-S on the expression of Sertoli cell genes important for testicular function; and 3) identify and initially characterize the P-Mod-S receptor. The completion of these goals will provide additional understanding of the paracrine factor P-Mod-S and its significance in the maintenance and control of spermatogenesis. Thus, further investigation of the testicular paracrine factor P-Mod-S, will provide information needed for the potential future design of agents to act on the testis as a contraceptive or for the prevention of infertility.

睾丸体细胞在维持和发育过程中发挥着不可或缺的作用。 精子发生的控制。体细胞-细胞相互作用的分析 已经证明了睾丸小管周细胞 分泌P-MOD-S，一种局部来源的、非促有丝分裂的旁分泌因子。P-MOD-S 支持细胞的功能维持生发细胞的发育。P-MOD-S 已经被证明对支持细胞的刺激程度比任何 已知的影响细胞的单一调节剂。因此， P-MOD-S介导的肾小管周围细胞-支持细胞相互作用 被认为在维护和控制 睾丸功能。本研究方案的目标是：1)确定 P-MOD-S对支持细胞作用机制的研究 P-MOD-S对支持细胞重要基因表达的影响 睾丸功能；3)P-MOD-S的鉴定和初步表征 受体。这些目标的完成将提供额外的 对旁分泌因子P-Mod-S的认识及其在糖尿病发病中的意义 精子发生的维持和控制。因此，进一步调查 睾丸旁分泌因子P-莫德-S将提供所需信息 对于潜在的未来设计的代理作为一个作用于睾丸 避孕或用于预防不孕症。