IMMUNOINCOMPETENT RODENT AND BIOHAZARD FACILITY

免疫功能低下的啮齿动物和生物危害设施

• 批准号: 7764706
• 负责人: JOHN N. NORTON
• 金额: $ 23.78万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7764706
• 关键词: Accreditation; Air; Air Conditioning; Animals; Area; Biohazardous Substance; Boxing; Cancer Biology; Cancer Center; Caring; Catheters; Charge; Containment; Containment of Biohazards; Doctor of Medicine; Doctor of Philosophy; Doctor of Veterinary Medicine; Duke Comprehensive Cancer Center; Ensure; Equipment; Fees; Gnotobiotic; Grant; Guidelines; Housing; Hybridomas; Image; Immunochemistry; Laboratories; Laboratory Animals; Lead; Maintenance; Malignant Neoplasms; Mechanics; Nude Mice; Nude Rats; Operating System; Operative Surgical Procedures; Positioning Attribute; Procedures; Production; Radiolabeled; Rattus; Recombinant DNA; Recommendation; Recruitment Activity; Research Personnel; Research Support; Resource Sharing; Resources; Rodent; Site Visit; Specific Pathogen Frees; System; Universities; Veterinarians; Work; animal care; animal resource; authority; biocontainment facility; cancer research center director; cost; design; germ free condition; immunosuppressed; implantation; improved; programs; radiotracer; research study; square foot; tumor

IMMUNOINCOMPETENT RODENT AND BIOHAZARD FACILITY John Norton, D.V.M., Ph.D., Director and Dareil Bigner, M.D., Ph.D., Scientific Director The Cancer Center Isoaltion Facility(CCIF) which houses the Immunoincompetent Rodent and Biocontainment Facility is the oldest shared resource in the Duke Comprehensive Cancer Center. This facility provides Cancer Center investigators with special isolated laboratories for work with hazardous biomolecules, certain recombinant DNA procedures, and for the maintenance of immunosuppressed and specific pathogen-free rodents or for other experiments that require biosafety level 2, 3, & 4 containment needed to investigate cancer biology and treatment. The building has a total of 30,000 square feet which includes eight modules housing 3 lead lined rooms, 3 horsfall rooms, 23 animal holding rooms, 9 laboratories, support areas for mechanical equipment, cage washing equipment and 26 operational autoclaves. Request for use of the space in the IRBF is evaluated by a user's committee which makes recommendations to the Director of the Cancer Center who has the authority to assign space in the building. Each investigator is assessed a fee for the use of the facility to recover the cost of staffing. The facility supports the research of 24 investigators and operates at full capacity. Dr.John N. Norton, Director of the Division of Laboratory Animal Resources and the operations of the IRBF, has the responsibility of managing and overseeing the animal care program. Dr. Darell Bigner is the scientific Coordinator of the IRBF. Mark Dewhirst, DVM, PhD will be the director of the new Animal Imaging Systems. The CCIF is staffed with 12 technicians and an operations manager who are responsible for providing care to the 4000 cages of rodents which must be maintained under barrier or containment conditions provided by the design of the building. The facility is supported by 4 fulltime mechanics paid from institutional resources to maintain and ensure the mechanical systems operate within biohazard containment guidelines. The CC1F provides space to support the Hybridoma/Immunochemistry and Radiolabeling Shared Resources.

免疫缺陷鼠与生物危害因素 约翰·诺顿，D.V.M.，哲学博士、导演和Dareil Bigner，医学博士，哲学博士、科学主任 癌症中心隔离设施（CCIF）容纳免疫活性啮齿动物， 生物防护设施是杜克综合癌症中心最古老的共享资源。这 该设施为癌症中心的研究人员提供了特殊的隔离实验室，用于危险的 生物分子，某些重组DNA程序，以及用于维持免疫抑制和 无特定病原体的啮齿动物或其他需要生物安全等级2、3和4的实验 需要研究癌症生物学和治疗。该建筑总面积为30，000平方英尺， 包括8个模块，其中包含3个铅内衬室，3个马槽室，23个动物饲养室，9个实验室， 机械设备、笼舍清洗设备和26个操作高压灭菌器的支持区。请求 IRBF中空间的使用由用户委员会评估，该委员会向 癌症中心主任，有权分配大楼内的空间。每一位研究者都是 评估了使用该设施的费用，以收回工作人员的费用。该设施支持24项研究 调查员和全面运作。John N. Norton，实验动物部主任 IRBF的资源和运营，负责管理和监督动物护理 程序. Darell Bigner博士是IRBF的科学协调员。马克Dewhirst，DVM，博士将是 新动物成像系统的主管CCIF配备了12名技术人员和一名操作人员。 负责照顾4000个鼠笼的管理人员， 建筑物设计提供的屏障或密封条件。该设施由4名全职人员提供支持 由机构资源支付的机械师，以维护和确保机械系统运行 在生物危害控制指南内。CC 1F提供了空间来支持 杂交/免疫化学和放射性标记共享资源。