IMMUNOINCOMPETENT RODENT AND BIOHAZARD FACILITY

免疫系统--啮齿动物和生物危害设施 John Norton，D.V.M.，博士，主任；Dareil Bigner，医学博士，博士，科学主任 癌症中心等离子设施(CCIF)，它容纳了免疫能力不强的啮齿动物和 生物遏制设施是杜克综合癌症中心最古老的共享资源。这 设施为癌症中心研究人员提供专门的隔离实验室，用于危险工作 生物分子，某些重组DNA程序，以及用于维持免疫抑制和 无特定病原体的啮齿动物或需要生物安全等级2、3和4的其他实验 需要研究癌症生物学和治疗。该建筑总面积为3万平方英尺， 包括8个模块，包括3个带铅衬里的房间、3个落马房间、23个动物拘留室、9个实验室、 支持机械设备、笼子清洗设备和26台运行中的高压灭菌器。申请 IRBF中空间的使用由用户委员会进行评估，该委员会向 癌症中心主任，有权分配大楼内的空间。每个调查员都是 评估了使用该设施的费用，以收回人员编制费用。该设施支持24人的研究 调查人员，并满负荷运作。约翰·N·诺顿博士，实验动物部主任 IRBF的资源和运作，负责管理和监督动物护理 程序。达雷尔·比格纳博士是IRBF的科学协调员。马克·德惠斯特，DVM，博士将成为 新的动物成像系统的主管。CCIF有12名技术人员和一名操作员 负责照顾4000笼老鼠的经理，这些笼子必须维持在 建筑物设计提供的屏障或围堵条件。该设施由4名全职人员提供支持 机械师从机构资源中支付费用，以维护和确保机械系统运行 在生物危害控制指南范围内。CC1F提供空间来支持 杂交瘤/免疫化学和放射性标记共享资源。

IMMUNOINCOMPETENT RODENT AND BIOHAZARD FACILITY John Norton, D.V.M., Ph.D., Director and Dareil Bigner, M.D., Ph.D., Scientific Director The Cancer Center Isoaltion Facility(CCIF) which houses the Immunoincompetent Rodent and Biocontainment Facility is the oldest shared resource in the Duke Comprehensive Cancer Center. This facility provides Cancer Center investigators with special isolated laboratories for work with hazardous biomolecules, certain recombinant DNA procedures, and for the maintenance of immunosuppressed and specific pathogen-free rodents or for other experiments that require biosafety level 2, 3, & 4 containment needed to investigate cancer biology and treatment. The building has a total of 30,000 square feet which includes eight modules housing 3 lead lined rooms, 3 horsfall rooms, 23 animal holding rooms, 9 laboratories, support areas for mechanical equipment, cage washing equipment and 26 operational autoclaves. Request for use of the space in the IRBF is evaluated by a user's committee which makes recommendations to the Director of the Cancer Center who has the authority to assign space in the building. Each investigator is assessed a fee for the use of the facility to recover the cost of staffing. The facility supports the research of 24 investigators and operates at full capacity. Dr.John N. Norton, Director of the Division of Laboratory Animal Resources and the operations of the IRBF, has the responsibility of managing and overseeing the animal care program. Dr. Darell Bigner is the scientific Coordinator of the IRBF. Mark Dewhirst, DVM, PhD will be the director of the new Animal Imaging Systems. The CCIF is staffed with 12 technicians and an operations manager who are responsible for providing care to the 4000 cages of rodents which must be maintained under barrier or containment conditions provided by the design of the building. The facility is supported by 4 fulltime mechanics paid from institutional resources to maintain and ensure the mechanical systems operate within biohazard containment guidelines. The CC1F provides space to support the Hybridoma/Immunochemistry and Radiolabeling Shared Resources.