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Rodent Cancer Models Core

啮齿动物癌症模型核心

基本信息

批准号：
10323311
负责人：
JOHN N. NORTON
金额：
$ 49.2万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-01-01 至 2024-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10323311
关键词：
Advanced Malignant Neoplasm Animal Barrier Facility Animal Housing Animal Model Animals Breeding Cancer Biology Cancer Center Cancer Center Support Grant Cancer Model Cell Line Censuses Clustered Regularly Interspaced Short Palindromic Repeats Consultations Custom Development Diagnosis Diagnostic Services Embryo Emerging Technologies Engineering Ensure Environment Equipment Gene Targeting Generations Genes Genetic Engineering Genetic Models Genome engineering Genomics Goals Hazardous Substances Heart Housing Human Human Resources Immunocompromised Host Inbreeding Individual Institutes Investigation Investments Journals Laboratory Animals Location Maintenance Malignant Neoplasms Mediating Modeling Molecular Analysis Mus New Agents Nude Mice Optics Pathology Plants Procedures Production Publications Reader Reagent Recombinant DNA Research Research Institute Research Personnel Research Training Resource Sharing Risk Robotics Rodent Rodent Model Services Sterility Students Synthetic Genes System Techniques Technology Testing Training Transgenic Organisms Update Water Work animal facility animal resource anticancer research biobank biosecurity cancer therapy cost cost effective design embryonic stem cell gene synthesis genome editing germ free condition immunosuppressed improved medical schools member molecular imaging operation pathogen programs protocol development research and development square foot user-friendly

项目摘要

ABSTRACT – RODENT CANCER MODELS SHARED RESOURCE The Rodent Cancer Models Shared Resource (RCM) provides Duke Cancer Institute (DCI) members a full service approach to the investigation of cancer biology, from the creation of rodent models through the testing of chemotherapeutics. To achieve that goal DCI’s RCM combined two primary services: the Cancer Center Isolation Facility (CCIF) and the Rodent Genetic Engineering Services Team (RGEST). CCIF is a 30,000 square foot, stand-alone rodent barrier facility located in the heart of the Duke biomedical campus operated by the Duke Division of Laboratory Animal Resources (DLAR) and dedicated to cancer research. The CCIF physical plant supports work with hazardous materials at biosafety level 2 (BSL2), recombinant DNA, chemotherapeutics, as well as the maintenance of immunosuppressed (outbred athymic nude mice and inbred Nod SCID gamma (NSG) mice) and specific pathogen-free (SPF) rodents. Occupying space in CCIF and in nearby facities, RGEST produces custom designed, genetically altered, transgenic and gene-targeted mice. RCM personnel assist DCI investigators who require assistance with breeding services, veterinary or diagnostic services, animal and experimental protocol development, inoculation or testing of cell lines, and guidance for conducting safe and efficient cancer biology research. CCIF also provides equipment and rodent housing space for the CCSG- supported Optical Molecular Imaging and Analysis (OMIA) shared resource. Recently, significant investments by the Duke School of Medicine have enabled upgrades to CCIF and RGEST equipment. In 2018, the RCM provided services to 78 investigators, 61% of whom were DCI members and who accounted for 94% of total usage and represented all 8 DCI Research Programs. Use of this shared resource by DCI Members contributed to 332 DCI publications over the project period, 103 of which were in high impact journals (Impact Factor>9), demonstrating the value of RCM services. The immediate and long-term objectives of the RCM are to continue to provide high-quality barrier facility services, low-cost, high-quality immunocompromised and SPF mice and genetically engineered animal models using cutting edge approaches, improve and upgrade services when possible, and to provide key expertise and services to DCI members studying cancer biology and developing new agents for treating or diagnosing human cancers.

摘要-啮齿动物癌症模型共享资源啮齿动物癌症模型共享资源（RCM）为杜克癌症研究所（DCI）成员提供了一个完整的癌症生物学研究的服务方法，从啮齿动物模型的建立到测试化疗药物的作用。为了实现这一目标，DCI的RCM结合了两个主要服务：癌症中心隔离设施（CCIF）和啮齿动物基因工程服务团队（RGEST）。CCIF是一个3万平方位于杜克生物医学园区中心的一个独立的啮齿动物屏障设施，由杜克经营实验动物资源部（DLAR），致力于癌症研究。CCIF物理工厂支持生物安全2级（BSL 2）危险材料、重组DNA、化疗药物的工作，以及维持免疫抑制（远交无胸腺裸小鼠和近交Nod SCID γ（NSG））小鼠）和无特定病原体（SPF）啮齿动物。占用CCIF和附近设施的空间，RGEST 生产定制设计的、基因改变的、转基因的和基因靶向的小鼠。RCM人员协助DCI 需要协助育种服务、兽医或诊断服务、动物和实验方案开发、细胞系的接种或检测，以及进行安全和有效的癌症生物学研究。CCIF还为CCSG提供设备和啮齿动物住房空间- 支持光学分子成像和分析（OMIA）共享资源。最近，重大投资由杜克医学院进行的研究已经实现了CCIF和RGEST设备的升级。 2018年，RCM为78名调查人员提供了服务，其中61%是DCI成员，占总使用量的94%，代表了所有8个DCI研究项目。共享资源的使用在项目期间，DCI成员为332份DCI出版物做出了贡献，其中103份具有很高的影响力期刊（影响因子>9），展示了RCM服务的价值。近期和长期目标的RCM是继续提供高品质的屏障设施服务，低成本，高品质免疫功能低下和SPF小鼠和基因工程动物模型，在可能的情况下改进和升级服务，并为DCI成员提供关键的专业知识和服务研究癌症生物学和开发用于治疗或诊断人类癌症的新药剂。