Central Vivarium Sterilizer Installation

中央饲养室灭菌器安装

• 批准号: 8711607
• 负责人: JOHN N. NORTON
• 金额: $ 50万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8711607
• 关键词: Affect; Agriculture; American; Animal Experimentation; Animal Housing; Animal Model; Animals; Area; Basic Science; Biomedical Engineering; Businesses; Canis familiaris; Cardiovascular system; Commit; Communicable Diseases; Companions; Decontamination; Diagnostic; Direct Costs; Disease Outbreaks; Ensure; Equipment; Floor; Funding; Grant; Growth; Hazardous Substances; Health; Housing; Human; Infection Control; Investments; Laboratories; Laboratory Animals; Life; Maintenance; Mechanics; Movement; Mus; Neurosciences; Operative Surgical Procedures; Process; Quarantine; Rattus; Recovery; Research; Research Personnel; Resources; Risk; Rodent; Sanitation; Side; Site; Soil; Sterilization; Sterilization for infection control; Time; Translational Research; Translations; Travel; United States National Institutes of Health; Universities; Update; Work; aged; animal colony; animal facility; animal resource; design; ergonomics; experience; improved; medical schools; meetings; nonhuman primate; operation; pathogen; prevent; programs; repaired; sound; square foot; water conservation

DESCRIPTION (provided by applicant): Duke University is committed to scientific research and its translation to improve human health, including appropriate use of animals in research. The purpose of this proposal is to replace and relocate an aged autoclave located within Duke's Central Vivarium and reallocate space to optimally provide defined quarantine/isolation and animal receiving areas, and to provide the ability to decontaminate suspect caging and equipment from satellite areas. Eighty eight per cent of all mice and rats on campus are housed in barrier areas or facilities that are specifically designed to preclude entry and spread of adventitious agents that may negatively influence research objectives, and non-rodent animals are localized primarily within the Central Vivarium. The specific aims of this proposal essentially impact all animal researchers at Duke through the quarantine and isolation of 'suspect' animals and the minimizing of cross- contamination potential of infectious animals. Funds from this renovation grant are crucial to: a) replacing a 43-year- old autoclave that is necessary for maintenance of housing for isolation and quarantine in the Central Vivarium as well as for decontamination of caging and equipment from satellite facilities when rodent infectious disease outbreaks occur and b) to provide a quarantine/isolation area that is separate from the general animal housing area. Direct costs from the National Institutes of Health provide about $120 million of funding annually to animal research at Duke. Opened in 1973, the Central Vivarium is the main campus vivarium and remains structurally and operationally sound. This 29,129 net square feet (nsf) facility is arranged on two floors: a small ground floor area houses administrative and diagnostic laboratory space, with animal space located on the first floor. The Central Vivarium can house any laboratory animal species in Duke's program and is the main site for housing and use of USDA-regulated species, including non-human primates, canines, and agricultural species. Caging and equipment are processed in the Central Vivarium cagewash area on the north side of the facility and in a single corridor operation: soiled caging and equipment are removed from the animal rooms and transported into the "dirty side" of cagewash via a single corridor, clean caging and equipment exit mechanical washers directly into the clean side of cagewash. Construction funded through C06 grants from the NIH utilizing American Reinvestment and Recovery Act (ARRA) resources is underway to build 2,731 nsf to meet the needs of biomedical engineering researchers that require specialized procedural areas and large animal models as well as a two-story addition to the Central Vivarium of about 19,000 nsf. The latter project will provide housing and procedural space for USDA-regulated species (e.g., nonhuman primates, agricultural and companion species) to accommodate the growth Duke is experiencing in neuroscience, cardiovascular, surgical, biomedical engineering, and translational research. The Central Vivarium also supports a number of rodent satellite facilities that lack the ability to sanitize caging and equipment as well as autoclaving of these items. Caging and equipment from these facilities are transported to the Central Vivarium for decontamination via the sterilizer and subsequent sanitation in the cagewash area. The acquisition and relocation of a new sterilizer, along with refurbishment of an area for quarantine/isolation will improve decontamination of materials, reduce the chance of adventitious agent outbreaks, and enhance the connections of the original Central Vivarium to the C06 grant-funded Central Vivarium additions currently underway.

描述（由申请人提供）：杜克大学致力于科学研究及其翻译，以改善人类健康，包括在研究中适当使用动物。本提案的目的是更换和重新安置位于杜克中心动物饲养场内的老化高压灭菌器，并重新分配空间，以最佳方式提供规定的检疫/隔离和动物接收区域，并提供对卫星区域的可疑笼舍和设备进行去污的能力。校园内88%的小鼠和大鼠被安置在屏障区或专门设计的设施中，以防止可能对研究目标产生负面影响的外来因子的进入和传播，而非啮齿动物主要位于中央动物园内。这项提案的具体目标基本上是 通过对“可疑”动物的检疫和隔离以及最大限度地减少传染性动物的交叉污染可能性，影响杜克的所有动物研究人员。来自该翻新补助金的资金对于以下方面至关重要：a）更换43年历史的高压灭菌器，该高压灭菌器对于维护中央动物饲养场的隔离和检疫所需，以及在啮齿动物传染病爆发时对笼舍和卫星设施的设备进行去污是必要的，以及B）提供与一般动物饲养区分开的检疫/隔离区。来自美国国立卫生研究院的直接费用每年为杜克的动物研究提供约1.2亿美元的资金。中央动物园于1973年开放，是校园的主要动物园，结构和运作良好。这个29，129净平方英尺（NSF）的设施分为两层：一层是行政和诊断实验室空间，一层是动物空间。中央动物园可以容纳杜克项目中的任何实验动物物种，并且是美国农业部监管物种（包括非人灵长类动物、犬科动物和农业物种）的主要饲养和使用场所。在设施北侧的Central Vivarium笼洗区和单一走廊操作中处理笼舍和设备：将污染的笼舍和设备从动物房中取出，并通过单一走廊运输到笼洗的“脏侧”，清洁的笼舍和设备从机械清洗机中直接排出，进入笼洗的清洁侧。通过美国国立卫生研究院利用美国再投资和恢复法案（ARRA）资源的C 06赠款资助的建设正在进行中，以建造2，731个nsf，以满足生物医学工程研究人员的需求，这些研究人员需要专门的程序区域和大型动物模型以及两层楼的中央动物园，约19，000个nsf。后一个项目将为美国农业部监管的物种提供住房和程序空间（例如，非人类灵长类动物、农业和伴侣物种），以适应杜克在神经科学、心血管、外科、生物医学工程和转化研究方面的增长。中央动物园还支持一些啮齿动物卫星设施，这些设施缺乏对笼子和设备进行消毒以及对这些物品进行高压灭菌的能力。将这些设施中的笼舍和设备运输至中央动物园，通过灭菌器进行去污，并随后在笼洗区进行卫生处理。新灭菌器的购置和搬迁，沿着检疫/隔离区的翻新，将改善材料的去污，降低外源因子爆发的可能性，并加强原中央动物饲养场与目前正在进行的C 06赠款资助的中央动物饲养场的连接。