RETROVIRUSES AND CANCER

逆转录病毒和癌症

• 批准号: 3093224
• 负责人: PAUL Eric NEIMAN
• 金额: $ 61.05万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-07-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3093224
• 关键词: Retroviridae; Retroviridae disease; oncogenic virus; viral leukemogenesis; virus genetics

The central theme of this program is the role of retroviruses and retrovirus-related oncogenes in neoplastic change. The grant consists of seven individual projects which address the issue across a broad front. Project 1 is concerned with the location and function within the cell of tyrosine protein kinases encoded by a number of viral oncogenes and the molecules which serve as the substrates for these enzymes. Project 2 attempts to exploit the suppression of gene expression by anti-sense RNA to determine whether this strategy can be used to manipulate the expression of c-myc and c-ras oncogenes. Project 3 will explore the role of tyrosine protein kinases in the action of polypeptide hormones on differentiation and cell growth. Project 4 will involve purification and microinjection analysis of the protein of the myc oncogene. Project 5 concerns the chromatin structure of the myc oncogene and effects of retroviral integration events on myc chromatin structure and expression. Project 6 will examine the role of specific domains of the v-myc gene in the oncogenicity of avian myelocytomatosis virus. Project 7 will attempt to determine the role of two oncogenes, myc and Blym-1, in specific stages of virus induced lymphomagenesis in the bursa of Fabricius in chickens.

该计划的中心主题是逆转录病毒的作用， 逆转录病毒相关癌基因在肿瘤变化中的作用。 补助金包括 七个单独的项目，解决了广泛的问题。 项目1涉及细胞内的位置和功能， 由许多病毒癌基因编码的酪氨酸蛋白激酶， 作为这些酶的底物的分子。 计划2 试图利用反义RNA对基因表达的抑制， 确定此策略是否可用于操纵 c-myc和c-ras癌基因。 项目3将探索酪氨酸的作用 多肽激素对分化作用中的蛋白激酶 和细胞生长。 项目4将涉及纯化和显微注射 myc癌基因的蛋白质分析。 项目5涉及 myc癌基因的染色质结构和逆转录病毒的作用 myc染色质结构和表达的整合事件。 Project 6 将研究v-myc基因的特定结构域在 禽骨髓细胞瘤病病毒的致癌性 第7章试图 确定两个癌基因myc和Blym-1在特定阶段的作用， 病毒诱导的鸡法氏囊淋巴瘤发生。