Workshop On Experimental Models from Bursa of Fabricius

法布里修斯布尔萨实验模型研讨会

• 批准号: 6561382
• 负责人: PAUL Eric NEIMAN
• 金额: $ 0.5万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6561382
• 关键词: B lymphocyte; bursa of Fabricius; disease /disorder model; neoplasm /cancer genetics; neoplasm /cancer immunology; travel; workshop

DESCRIPTION (provided by applicant): The workshop on Experimental Models from the Bursa of Fabricius will be held October 4-6, 2002, in the modern conference facilities of a leading biomedical research institute, the Fred Hutchinson Cancer Research Center, in Seattle, Washington. Experimental model systems based on the bursa and its derivatives provide highly productive opportunities for study of biological problems of broad significance. Examples include: (1) pathogenesis of neoplasia induced by oncogenes such as c-myc, c-myb and Rel, including analysis of angiogenesis and programmed cell death. (2) B-cell development, including mechanisms of immunoglobulin gene diversification, (3) somatic stem cell biology and transplantation, (4) targeted gene deletion and replacement in cultured bursal-derived (DT40) cells. This latter technology, unique for use with cultured vertebrate cell lines, has been exploited for novel and revealing experiments in an increasing range of applications including; recombination and conversion in lg gene diversification, signal transduction pathways from B-cell receptors, regulation of RNA splicing, function of centromeric proteins in chromosome dynamics and targeted mutagenesis in human chromosomes shuttled in and out of DT40 cells. Despite such achievements these experimental approaches are not as widely known and applied as might be expected from the opportunity they represent. One limitation has been a lack of chicken genomic resources for both construction and analysis of experiments in the bursa and DT40 cells. Therefore, the workshop will feature (5) the expanded array of genomic resources now available and/or under development for use in these systems. The workshop will bring together, for the first time, leading investigators working with these related experimental systems in order to meet each other and share their findings and expertise. A core of 17 leading investigators are invited speakers. The remaining program of speakers and posters will be selected from submitted abstracts in response to a general announcement. The meeting will be open to interested students post-doctoral trainees and scientists up to the capacity of the facilities. This workshop will serve to stimulate further development and application, and to educate interested young scientists to the opportunities presented.

描述（由申请人提供）：法氏囊实验模型研讨会将于2002年10月4日至6日在华盛顿西雅图的一家领先的生物医学研究机构弗雷德哈钦森癌症研究中心的现代会议设施举行。 基于囊及其衍生物的实验模型系统为具有广泛意义的生物学问题的研究提供了高产的机会。 示例包括：（1）c-myc、c-myb、Rel等癌基因诱发肿瘤的发病机制，包括血管生成和程序性细胞死亡的分析。(2)B细胞发育，包括免疫球蛋白基因多样化的机制，（3）体干细胞生物学和移植，（4）培养的囊源（DT 40）细胞中靶向基因的缺失和替换。 后一种技术，独特的用于培养的脊椎动物细胞系，已被开发用于新的和揭示性的实验，在越来越多的应用范围，包括：重组和转换的Ig基因多样化，信号转导途径从B细胞受体，RNA剪接的调节，着丝粒蛋白质的功能，在染色体动力学和靶向诱变穿梭在DT 40细胞的人类染色体。 尽管取得了这些成就，但这些实验性方法并没有像它们所代表的机会所预期的那样广为人知和得到广泛应用。 一个限制是缺乏鸡基因组资源的建设和分析的实验，在囊和DT 40细胞。 因此，研讨会将重点介绍（5）现有和/或正在开发的用于这些系统的基因组资源的扩展阵列。 该研讨会将首次汇集与这些相关实验系统合作的主要研究人员，以便相互见面并分享他们的研究结果和专业知识。 17名主要研究人员的核心应邀发言。 其余的演讲者和海报计划将从提交的摘要中选出，以回应一般性公告。 会议将向感兴趣的学生、博士后学员和科学家开放，但以设施的能力为限。 该讲习班将有助于促进进一步的开发和应用，并教育感兴趣的年轻科学家利用所提供的机会。