PROGRAM IN RETROVIRUSES AND CANCER

该计划的中心主题是逆转录病毒和 肿瘤变化中逆转录病毒相关的癌基因。 该补助金包括 七个单独的项目在广泛的领域解决了这个问题。 项目 1 涉及细胞内的位置和功能 由许多病毒癌基因编码的酪氨酸蛋白激酶和 作为这些酶的底物的分子。 项目2 试图利用反义RNA对基因表达的抑制来 确定该策略是否可用于操纵 c-myc 和 c-ras 癌基因。 项目3将探索酪氨酸的作用 蛋白激酶在多肽激素分化作用中的作用 和细胞生长。 项目4将涉及纯化和显微注射 myc癌基因的蛋白质分析。 项目 5 涉及 myc癌基因的染色质结构和逆转录病毒的影响 myc 染色质结构和表达的整合事件。 项目6 将检查 v-myc 基因特定域在 禽骨髓细胞瘤病毒的致癌性。 项目 7 将尝试 确定两个癌基因 myc 和 Blym-1 在特定阶段的作用 病毒诱导鸡法氏囊发生淋巴瘤。

The central theme of this program is the role of retroviruses and retrovirus-related oncogenes in neoplastic change. The grant consists of seven individual projects which address the issue across a broad front. Project 1 is concerned with the location and function within the cell of tyrosine protein kinases encoded by a number of viral oncogenes and the molecules which serve as the substrates for these enzymes. Project 2 attempts to exploit the suppression of gene expression by anti-sense RNA to determine whether this strategy can be used to manipulate the expression of c-myc and c-ras oncogenes. Project 3 will explore the role of tyrosine protein kinases in the action of polypeptide hormones on differentiation and cell growth. Project 4 will involve purification and microinjection analysis of the protein of the myc oncogene. Project 5 concerns the chromatin structure of the myc oncogene and effects of retroviral integration events on myc chromatin structure and expression. Project 6 will examine the role of specific domains of the v-myc gene in the oncogenicity of avian myelocytomatosis virus. Project 7 will attempt to determine the role of two oncogenes, myc and Blym-1, in specific stages of virus induced lymphomagenesis in the bursa of Fabricius in chickens.