MOLECULAR AND DEVELOPMENTAL GENETICS OF DROSOPHILA

果蝇的分子和发育遗传学

• 批准号: 3096103
• 负责人: FOTIS C KAFATOS
• 金额: $ 99.74万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3096103
• 关键词: biomedical facility; developmental genetics; molecular genetics

We are involved in studying the structure and regulation of eukaryotic genes, using Drosophila as an experimental object. Although we come from diverse background (genetics, molecular biology, developmental biology, population genetics, cell biology), we intersect in our common interest: to use the genetically most favorable higher organism, Drosophila, and the current techniques of molecular and cellular biology, to illuminate how the genes of higher are organized, evolve, and are regulated during development and during the cell cycle. Indeed, a strength of our group is that it represents complementary and yet overlapping approaches. In the context of the program project, we intend to: (a) Test by genetics the in vivo function of transcription factors putatively involved in the developmental regulation of chorion genes, and isolate additional components involved in chorion regulation using enhancer trap method. (b) Study and manipulate the hobo mobile elements, including characterization of the transposase transcript and the mechanism of transposition of the element in the germ line; develop novel transformation vector and procedures. (c) Characterize DNA polymorphism and evolution of the decapentaplegic gene in populations of D. melanogaster and D. pseudoobscura, to obtain the first data on selective constraints on amino acid sequences, message structure and cis-regulatory elements for a gene controlling basic morphogenetic processes. (d) Use genetic approaches to understanding the control of embryonic muscle cell commitment and differentiation, focusing on the nau gene that encodes basic and helix-loop-lelix domains similar to those of vertebrate myogenic regulatory factors. (e) Study the in vitro properties and in vivo function of the products of two genes, ned and nod, which belong to the kinesin superfamily and movements of microtubules in the meiotic and mitotic spindle apparatus.

我们参与研究真核生物的结构和调控， 基因，使用果蝇作为实验对象。 虽然我们来自 不同的背景（遗传学，分子生物学，发育生物学， 群体遗传学、细胞生物学），我们在共同的兴趣上相交： 使用遗传上最有利的高等生物果蝇， 目前的分子和细胞生物学技术，以阐明如何 高等植物的基因在发育过程中被组织、进化和调节， 以及细胞周期中的变化。 其实，我们的一个优势是， 代表了互补但又重叠的方法。 背景下 该计划项目，我们打算： (a)通过遗传学检测转录因子的体内功能 参与绒毛膜基因发育调节的puerectin，和 使用增强子分离涉及绒毛膜调节的其它组分 陷阱法 (b)研究和操纵流浪汉移动的元素，包括 转座酶转录物的表征和 转座的元素在生殖细胞系;开发新的转化 矢量和程序。 (c)描述DNA多态性和十肢瘫痪基因的进化 在D.黑腹果蝇D. pseudobscura，以获得第一个 关于氨基酸序列的选择性约束、信息结构的数据 和控制基本形态发生的基因的顺式调节元件 流程. (d)利用遗传学方法了解胚胎肌肉的控制 细胞定型和分化，重点是nau基因，编码 与脊椎动物肌源性的那些相似的碱性和螺旋-环-LIPE结构域 调节因素。 (e)研究其产物的体外性质和体内功能 ned和nod两个基因属于驱动蛋白超家族， 微管在减数分裂和有丝分裂纺锤体中的运动。