MOLECULAR AND DEVELOPMENTAL GENETICS OF DROSOPHILA

果蝇的分子和发育遗传学

• 批准号: 3096104
• 负责人: FOTIS C KAFATOS
• 金额: $ 75.17万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-08-01 至 1986-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3096104
• 关键词: developmental genetics; genetic manipulation

We are involved in studying the structure and regulation of eukaryotic genes, using Drosophila as an experimental object. Although we come from diverse backgrounds, (genetics, molecular biology, development biology, population genetics), we intersect in our common interest: to use the genetically most favorable higher organism, Drosophila, and the current techniques of cellular and molecular biology, to illuminate how the genes of higher organisms are organized, evolve, and are regulated during development. Indeed, a strength of our group is that it represents complementary and yet overlapping approaches. In the context of a program project, we intend to: (a) Analyze by molecular and genetic means the mechanisms regulating the orderly production of chorion proteins during oogenesis. (b) Study in vitro transcription of chorion and ecdysteroid-induced genes, and the regulation of the latter by steroids. (c) Analyze by molecular and cytogenetic techniques an interesting chromosomal region, 71C-E, which encompasses ecdysteroid-induced genes. (d) Develop techniques for somatic cell genetics, using continuous cell lines of defined genotype. (e) Study by molecular and genetic techniques the choline acetyltransferase gene and possible regulatory mutants affecting its expression. (f) Study genetic polymorphism by sequencing the alcohol dehydrogenase gene of isogenic lines derived from four natural populations. (g) Study the nature of large transposable elements.

我们参与研究真核生物的结构和调控， 基因，使用果蝇作为实验对象。 虽然我们来自 不同的背景，（遗传学，分子生物学，发育生物学， 人口遗传学），我们相交于我们共同的利益：使用 遗传上最有利的高等生物，果蝇，和电流 细胞和分子生物学技术，来阐明基因是如何 高等生物的组织、进化和调节， 发展 事实上，我们集团的一个优势是，它代表了 互补但重叠的方法。 在一个程序中， （a）通过分子和遗传手段分析 绒毛膜蛋白的有序产生的调节机制 卵子发生。 (b)绒毛膜细胞体外转录的研究 蜕皮激素诱导的基因，以及类固醇对后者的调节。 (c)通过分子和细胞遗传学技术分析， 染色体区域，71 C-E，其包括蜕皮激素诱导的基因。 (d)发展体细胞遗传学技术，利用连续细胞 确定的基因型。 (e)通过分子和遗传技术进行研究 胆碱乙酰转移酶基因和可能的调节突变体 影响其表达。 (f)通过测序研究遗传多态性 乙醇脱氢酶基因来自四个天然来源的等基因系 人口。 (g)研究大型转座因子的性质。