MOLECULAR AND DEVELOPMENTAL GENETICS OF DROSOPHILA

果蝇的分子和发育遗传学

• 批准号: 3096107
• 负责人: FOTIS C KAFATOS
• 金额: $ 80.39万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3096107
• 关键词: Drosophilidae; developmental genetics; molecular genetics

We are involved in studying the structure and regulation of eukaryotic genes, using Drosophila as an experimental object. Although we come from diverse backgrounds (genetics, molecular biology, developmental biology, population genetics, cell biology), we intersect in our common interest: to use the genetically most favorable higher organism, Drosophila, and the current techniques of molecular and cellular biology, to illuminate how the genes of higher organisms are organized, evolve, and are regulated during development and during the cell cycle. Indeed, a strength of our group is that it represents complementary and yet overlapping approaches. In the context of the program project, we intend to: (a) Analyze by molecular and genetic means the mechanisms regulating the orderly production of chorion proteins during oogenesis. (b) Investigate by molecular and genetic means the processes controlling transvection, position effects and somatic chromosome pairing. (c) Study genetic polymorphism by sequencing the alcohol dehydrogenase and xanthine dehydrogenase genes of isogenic lines derived from natural population of Drosophila pseudoobscura. (d) Characterize RNA Polymerase II mutants of Drosophila, and attempt to accomplish homologous transformation in the germ line. (e) Characterize structurally and developmentally a homeobox-containing maternal-effect gene, Hb38E, and pair-rule segmentation mutants that map in the same interval. (f) Study the structure and in vivo function of the 205K microtubule-associated protein, the gene for which we have cloned.

我们致力于研究真核生物的结构和调控。 基因，以果蝇为实验对象。虽然我们来自 不同的背景(遗传学、分子生物学、发育生物学、 人口遗传学、细胞生物学)，我们为了我们的共同利益而相交： 利用遗传上最有利的高等生物--果蝇和 目前的分子和细胞生物学技术，以阐明 高等生物体的基因在过程中被组织、进化和调节 在发育和细胞周期中。的确，我们集团的一个优势是 它代表了相辅相成但又重叠的方法。 在该方案项目的背景下，我们打算： (A)用分子和遗传手段分析调节 卵子发生过程中绒毛膜蛋白的有序产生。 (B)用分子和遗传手段研究控制过程 转基因、位置效应和体细胞染色体配对。 (C)通过对酒精脱氢酶进行测序来研究遗传多态 天然黄嘌呤脱氢酶基因等基因系的研究 拟黑腹果蝇种群。 (D)鉴定果蝇的RNA聚合酶II突变体，并尝试 在种质系中完成同源转化。 (E)从结构和发育上描述含有同源异型盒的 母性效应基因Hb38E和配对规则分段突变体 同样的时间间隔。 (F)研究205K的结构和体内功能 微管相关蛋白，我们为其克隆的基因。