MOLECULAR AND DEVELOPMENTAL GENETICS OF DROSOPHILA

果蝇的分子和发育遗传学

• 批准号: 3096102
• 负责人: FOTIS C KAFATOS
• 金额: $ 69.24万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3096102
• 关键词: developmental genetics; genetic manipulation

We are involved in studying the structure and regulation of eukaryotic genes, using Drosophila as an experimental object. Although we come from diverse backgrounds (genetics, molecular biology, developmental biology, population genetics, cell biology), we intersect in our common interest: to use the genetically most favorable higher organism, Drosophila, and the current techniques of molecular and cellular biology, to illuminate how the genes of higher organisms are organized, evolve, and are regulated during development and during the cell cycle. Indeed, a strength of our group is that it represents complementary and yet overlapping approaches. In the context of the program project, we intend to: (a) Analyze by molecular and genetic means the mechanisms regulating the orderly production of chorion proteins during oogenesis. (b) Investigate by molecular and genetic means the processes controlling transvection, position effects and somatic chromosome pairing. (c) Study genetic polymorphism by sequencing the alcohol dehydrogenase and xanthine dehydrogenase genes of isogenic lines derived from natural population of Drosophila pseudoobscura. (d) Characterize RNA Polymerase II mutants of Drosophila, and attempt to accomplish homologous transformation in the germ line. (e) Characterize structurally and developmentally a homeobox-containing maternal-effect gene, Hb38E, and pair-rule segmentation mutants that map in the same interval. (f) Study the structure and in vivo function of the 205K microtubule-associated protein, the gene for which we have cloned.

我们参与研究真核生物的结构和调控， 基因，使用果蝇作为实验对象。 虽然我们来自 不同的背景（遗传学，分子生物学，发育生物学， 群体遗传学、细胞生物学），我们在共同的兴趣上相交： 使用遗传上最有利的高等生物果蝇， 目前的分子和细胞生物学技术，以阐明如何 高等生物的基因在进化过程中被组织、进化和调节， 发育和细胞周期。 的确，我们集团的一个优势是 它代表了互补但又重叠的方法。 在该项目的背景下，我们打算： (a)通过分子和遗传学手段分析其调控机制， 在卵子发生过程中绒毛膜蛋白的有序产生。 (b)通过分子和遗传学手段研究控制 转染、位置效应和体细胞染色体配对。 (c)通过对乙醇脱氢酶和 天然来源的等基因系的黄嘌呤脱氢酶基因 Drosophila pseudobscura的照片 (d)描述果蝇RNA聚合酶II突变体的特征，并试图 在种系中完成同源转化。 (e)在结构上和发育上表征含有同源框的 母亲效应基因，Hb38E，和配对规则分割突变体，映射在 同样的间隔。 (f)研究205K的结构和体内功能 微管相关蛋白，我们已经克隆的基因。