A MECHANISM FOR ASSESSING NEURAL SPROUTING IN THE DORSAL HORN

这个项目的目标是测试轴突的假设 脊髓灰质背侧3层的神经支配 对部分失去神经的反应而萌发。要做到这一点，它是 有必要设计一种方法来计算这些神经元中的突触 片层。这是一个体视学问题，因为我们必须 来自2维数据的3维数据(突触数目 (电子显微镜照片)。本研究提出了一个标准 获得这些数字的体视学方法。在第一个 一年来，我们一直专注于测量 突触在后角裂开，并发现平均 不同的叶片长度不同。下一步是 确定突触数量。

The goal of this project is to test the hypothesis that axons innervating the dorsal 3 laminae of the spinal cord gray matter sprout in response to a partial denervation. To do this, it is necessary to devise a method for counting the synapses in these laminae. This is a stereological problem in that we must then get 3 dimensional data (numbers of synapses) from 2 dimensional data (electron micrographs). The present study presents a standard stereological method for obtaining these numbers. In the first year, we have concentrated on measuring the length of the synaptic cleft in the dorsal horn and have found that the average length is different in the different laminae. The next step is to determine synaptic numbers.