A MECHANISM FOR ASSESSING NEURAL SPROUTING IN THE DORSAL HORN

评估背角神经萌芽的机制

• 批准号: 3882007
• 负责人: RICHARD E. COGGESHALL
• 金额: --
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3882007
• 关键词: axon; denervation; dorsal column; electron microscopy; laboratory rat; nervous system regeneration; neuroanatomy; neurogenesis; spinal cord; synapses

The goal of this project is to test the hypothesis that axons innervating the dorsal 3 laminae of the spinal cord gray matter sprout in response to a partial denervation. To do this, it is necessary to devise a method for counting the synapses in these laminae. This is a stereological problem in that we must then get 3 dimensional data (numbers of synapses) from 2 dimensional data (electron micrographs). The present study presents a standard stereological method for obtaining these numbers. In the first year, we have concentrated on measuring the length of the synaptic cleft in the dorsal horn and have found that the average length is different in the different laminae. The next step is to determine synaptic numbers.

这个项目的目标是检验轴突 支配脊髓灰质的背侧3层 在部分去神经后发芽。 要做到这一点， 有必要设计一种方法来计算这些突触 纹层 这是一个体视学问题，因为我们必须得到 来自二维数据的三维数据（突触数量） （电子显微照片）。 本研究提出了一个标准 体视学方法获得这些数字。 上 今年，我们集中精力测量了 突触间隙的背角，并已发现，平均 不同纹层的长度不同。 下一步是 确定突触数量