MICROBIOLOGIC STUDIES

微生物学研究

• 批准号: 3854285
• 负责人: JOSEPH J ZAMBON
• 金额: --
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3854285
• 关键词: AIDS; Actinobacillus actinomycetemcomitans; antibacterial agents; antibiotics; child (0-11); communicable disease transmission; communicable diseases; dental disorder diagnosis; dental plaque; disease /disorder proneness /risk; enzyme linked immunosorbent assay; genetic markers; gingivitis; host organism interaction; human subject; immunofluorescence technique; isozymes; laboratory mouse; laboratory rabbit; laboratory rat; longitudinal human study; microorganism classification; microorganism immunology; molecular cloning; monoclonal antibody; nucleic acid probes; oral bacteria; periodontitis; periodontium disorder; serology /serodiagnosis

Previous cross-sectional and longitudinal studies performed on small numbers of subjects have implicated a few specific oral microorganisms such as B. gingivalis, B. intermedius, and A. (Haemophilus) actinomycetemcomitans, in the etiology of the different forms of human periodontal disease. Recent studies, suggest that 1.) only a small proportion of the population is infected with these pathogens; 2.) these microorganisms are transmissible among family members and; 3.) specific antigenic groups within these species may be particularly virulent. Consequently, we have formulated a new hypothesis on the nature of periodontal infections. We propose that periodontal disease develops secondary to subgingival infection with specific periodontal pathogens transmitted from an exogenous source, and that this infection constitutes a "risk factor" for periodontal disease. We propose to test this hypothesis by the following experimental approaches: First, determining the prevalence of periodontal pathogens in cross-sectional and longitudinal studies of a large adult sample using our recently developed species and serogroup- specific serodiagnostic reagents in rapid immunofluorescence assays and by DNA probe technology. Second, we will examine intrafamilial transmission of periodontal pathogens by molecular biological techniques including restriction fragment length polymorphism and by isozyme and antigenic analysis of bacterial isolates. Third, we will characterize the subgingival microflora in periodontitis patients with Acquired Immunodeficiency Syndrome by means of anaerobic culture in order to determine which microorganisms are important in the pathogenesis of their periodontal disease. Fourth, we will pursue serologic studies of the putative periodontal pathogens to pinpoint serogroups and corresponding antigens which are important in the etiology of human periodontal diseases. These studies will provide significant new information which will be useful in targeting high risk subjects for preventive therapy prior to the initiation of overt periodontal destruction.

先前进行的横断面和纵向研究 少数受试者涉及一些特定的口腔 微生物如B。gingivalis，B. B. intermedius和A. （嗜血杆菌）伴放线菌，在病原学的 不同形式的人类牙周病。 最近的研究， 建议1）。只有一小部分人口 感染了这些病原体; 2.）这些微生物 可在家庭成员中传播; 3.）特定抗原 这些物种中的群体可能特别致命。 因此，我们提出了一个新的假设， 牙周感染 我们认为牙周病 继发于龈下感染， 从外源性来源传播的牙周病原体，以及 这种感染构成牙周炎的“危险因素”， 疾病 我们建议通过以下实验来验证这一假设 方法：首先，确定牙周炎的患病率， 病原体的横向和纵向研究， 使用我们最近开发的物种和血清型的成人样本- 快速免疫荧光测定中的特异性血清诊断试剂 和DNA探针技术。 其次，我们将研究家庭内部 牙周致病菌的分子生物学传播 技术包括限制性片段长度多态性和 通过细菌分离物的同工酶和抗原性分析。 第三、 我们将描述牙周炎的龈下菌群特征， 获得性免疫缺陷综合征患者， 厌氧培养，以确定哪些微生物是 在牙周病的发病机制中起重要作用。 第四，我们将继续进行血清学研究， 牙周病原体，以查明血清型和相应的 在人类牙周病病因学中重要的抗原 疾病 这些研究将提供重要的新信息， 可用于针对高危受试者进行预防性治疗 在开始明显的牙周破坏之前。