Bacterial and plant factors that influence adhesion of enterohaemorrhagic E. coli and Salmonella enterica to salad leaves

影响肠出血性大肠杆菌和沙门氏菌对沙拉叶粘附的细菌和植物因素

基本信息

批准号：
BB/G014175/1
负责人：
Paul Hand
金额：
$ 53.06万
依托单位：
University of Warwick
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2009
资助国家：
英国
起止时间：
2009 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FG014175%2F1
关键词：
Bacterial plant factors influence adhesion

项目摘要

Until now, research into transmission of food-borne pathogens has largely focused on farm animal reservoirs. Although transmission of enteric pathogens via salad leaves is on the rise, only a few studies have addressed the molecular mechanisms used by human enteric bacterial pathogens to attach to plants. Likewise there have been few studies investigating variation between plant genotypes on bacterial adhesion and/or survival and those that have not addressed the genetic basis of any differences. In this project we aim to investigate how human bacterial pathogens attach to salad leaves (in particular lettuce). We will determine the effect of bacterial growth condition on the ability of the organism to attach to leaves. Similarly, we will determine the effect of plant growth conditions on susceptibility to bacterial attachment. Using genetic approaches, we will determine which bacterial genes are involved in leaf attachment and we will utilise a lettuce mapping population of recombinant inbred lines to initiate studies aimed at gaining an understanding of the genetic basis of a plant's susceptibility to bacterial adhesion. We will also determine survival and replication rates of Salmonella and E. coli on the different lettuce lines. The ultimate goal of our studies is to provide the underpinning knowledge of the interaction between the plant and enteric bacteria in the phyllosphere to allow the development of strategies to reduce the risk of crop contamination and to increase food safety.

到目前为止，对食物传播病原体的传播的研究主要集中在农场动物储层上。尽管通过沙拉叶片传播肠道病原体，但只有少数研究解决了人类肠细菌病原体与植物附着的分子机制。同样，很少有研究研究了植物基因型在细菌粘附和/或生存方面的变化，以及尚未解决任何差异的遗传基础的差异。在这个项目中，我们旨在研究人类细菌病原体如何附着在沙拉叶（特别是生菜）上。我们将确定细菌生长条件对生物体附着在叶子上的能力的影响。同样，我们将确定植物生长条件对细菌附着的敏感性的影响。使用遗传方法，我们将确定哪些细菌基因参与叶片附着，我们将利用生菜映射的重组近交系的群体来开始研究，以了解旨在了解植物对细菌粘附的遗传基础的了解。我们还将在不同的生菜线上确定沙门氏菌和大肠杆菌的存活率和复制率。我们研究的最终目的是提供有关植物与植物细菌之间相互作用的基础知识，以允许制定策略，以减少作物污染的风险并提高食品安全。