INFLAMMATION AND ALVEOLAR TYPE II EPITHELIAL CELLS

炎症和肺泡 II 型上皮细胞

• 批准号: 3943996
• 负责人: ROBERT J MASON
• 金额: --
• 依托单位: NATIONAL JEWISH HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3943996
• 关键词: acute disease /disorder; allergic pneumonitis; apolipoproteins; bleomycin; blood lipoprotein biosynthesis; cell adhesion; cell age; cell growth regulation; complementary DNA; cytotoxicity; diagnostic respiratory lavage; disease /disorder model; electron microscopy; extracellular matrix; fibroblasts; histochemistry /cytochemistry; human subject; hyperplasia; inflammation; lipid biosynthesis; lung alveolus; microscopy; monoclonal antibody; penicillamine; phospholipids; pulmonary surfactants; respiratory epithelium; respiratory toxin; surface antigens; tissue /cell culture; trachea

Alveolar type II cells are specialized epithelial cells that line the alveolus and synthesize and secrete pulmonary surface active material. Type II cells are thought to be the stem cell for epithelium and proliferation of type II cells is critical for the restoration of gas exchange units after alveolar injury. The hypothesis, on which this project is based, is that for the functions of type II cells are important in our ultimate understanding of interstitial lung disease. In addition, we hypothesize that the functional state of type II cells can be determined in part by analysis of individual components of surfactant in lavage. We plan to analyze the 34 kd apoprotein (apoprotein A) and the phospholipids in lavage from patients with idiopathic pulmonary fibrosis and controls and from animals treated with bleomycin. We will develop methods of maintaining human and rat type II cells in a differentiated state in primary culture. This aim is very important for discovering the cellular regulation of different components of surfactant. Specifically, we want to determine if the 34 kd apoprotein, saturated phosphatidylcholine, and phosphatidylglycerol are independently regulated in type II cells from adult animals. We plan to develop methods for isolating hyperplastic type II cells to see if these cells are responsible for the alteration in surfactant lipids found in patients with ILD. Finally, we will test to see if there are growth factors for type II cells in lavages from patients or animals with interstitial lung disease. The proposed studies will focus on the role of parenchymal lung cells in the development of interstitial lung disease.

肺泡 II 型细胞是排列在肺泡上的特殊上皮细胞 肺泡合成和分泌肺表面活性物质 材料。 II 型细胞被认为是干细胞 II 型细胞的上皮和增殖对于 肺泡损伤后气体交换单位的恢复。 这 该项目所基于的假设是对于函数 II 型细胞的研究对于我们最终理解 间质性肺疾病。 此外，我们假设 II 型细胞的功能状态可以部分由以下因素决定 分析灌洗液中表面活性剂的各个成分。 我们 计划分析 34 kd 脱辅基蛋白（apo Protein A）和 特发性肺结核患者灌洗液中的磷脂 纤维化和对照以及来自用博来霉素治疗的动物。 我们将开发维持人类和大鼠 II 型的方法 原代培养中处于分化状态的细胞。 这个目的非常 对于发现不同细胞的调节非常重要 表面活性剂的成分。 具体来说，我们想要确定是否 34 kd 脱辅基蛋白、饱和磷脂酰胆碱和 磷脂酰甘油在 II 型细胞中独立调节 来自成年动物。 我们计划开发隔离方法 增生性 II 型细胞，看看这些细胞是否负责 ILD 患者中发现的表面活性剂脂质的变化。 最后我们会测试一下是否有II型生长因子 间质性肺患者或动物灌洗液中的细胞 疾病。 拟议的研究将重点关注 肺实质细胞在间质性肺发育中的作用 疾病。