INFLAMMATION AND ALVEOLAR TYPE II EPITHELIAL CELLS

炎症和肺泡 II 型上皮细胞

• 批准号: 3879915
• 负责人: ROBERT J MASON
• 金额: --
• 依托单位: NATIONAL JEWISH HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3879915
• 关键词: allergic pneumonitis; apolipoproteins; bleomycin; blood lipoprotein biosynthesis; cardiolipins; cell growth regulation; cellular pathology; diagnostic respiratory lavage; drug adverse effect; extracellular matrix; fibronectins; gel electrophoresis; hormone regulation /control mechanism; human subject; hyperplasia; inflammation; laboratory rat; leukocyte activation /transformation; lipid biosynthesis; lung alveolus; lung disorder; membrane activity; membrane lipids; mitogens; molecular pathology; phosphatidylcholines; phosphatidylinositols; phospholipids; pulmonary fibrosis /granuloma; pulmonary surfactants; respiratory epithelium; silicates; tissue /cell culture

Alveolar type II cells are specialized epithelial cells that line the alveolus and synthesize and secrete pulmonary surface active material. Type II cells are thought to be the stem cell for epithelium and proliferation of type II cells is critical for the restoration of gas exchange units after alveolar injury. The hypothesis, on which this project is based, is that for the functions of type II cells are important in our ultimate understanding of interstitial lung disease. In addition, we hypothesize that the functional state of type II cells can be determined in part by analysis of individual components of surfactant in lavage. We plan to analyze the 34 kd apoprotein (apoprotein A) and the phospholipids in lavage from patients with idiopathic pulmonary fibrosis and controls and from animals treated with bleomycin. We will develop methods of maintaining human and rat type II cells in a differentiated state in primary culture. This aim is very important for discovering the cellular regulation of different components of surfactant. Specifically, we want to determine if the 34 kd apoprotein, saturated phosphatidylcholine, and phosphatidylglycerol are independently regulated in type II cells from adult animals. We plan to develop methods for isolating hyperplastic type II cells to see if these cells are responsible for the alteration in surfactant lipids found in patients with ILD. Finally, we will test to see if there are growth factors for type II cells in lavages from patients or animals with interstitial lung disease. The proposed studies will focus on the role of parenchymal lung cells in the development of interstitial lung disease.

肺泡II型细胞是排列在肺泡内的特化上皮细胞