TYPE II CELLS AND INTERSTITIAL LUNG DISEASE

II 型细胞和间质性肺疾病

• 批准号: 3858936
• 负责人: ROBERT J MASON
• 金额: --
• 依托单位: NATIONAL JEWISH HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3858936
• 关键词: bleomycin; cell growth regulation; cellular pathology; diagnostic respiratory lavage; extracellular matrix; gel electrophoresis; growth factor; human subject; hyperplasia; laboratory rat; lung alveolus; lung disorder; mitogens; molecular pathology; phosphatidylcholines; phosphatidylinositols; phospholipids; pulmonary fibrosis /granuloma; pulmonary surfactants; respiratory epithelium; silicates; tissue /cell culture

Alveolar type II cells are specialized epithelial cells that line the alveolus and synthesize and secrete pulmonary surface active material. Type II cells are thought to be the stem cell for epithelium and proliferation of type II cells is critical for the restoration of gas exchange units after alveolar injury. The hypothesis, on which this project is based, is that for the functions of type II cells are important in our ultimate understanding of interstitial lung disease. In addition, we hypothesize that the functional state of type II cells can be determined in part by analysis of individual components of surfactant in lavage. We plan to analyze the 34 kd apoprotein (apoprotein A) and the phospholipids in lavage from patients with idiopathic pulmonary fibrosis and controls and from animals treated with bleomycin. We will develop methods of maintaining human and rat type II cells in a differentiated state in primary culture. This aim is very important for discovering the cellular regulation of different components of surfactant. Specifically, we want to determine if the 34 kd apoprotein, saturated phosphatidylcholine, and phosphatidylglycerol are independently regulated in type II cells from adult animals. We plan to develop methods for isolating hyperplastic type II cells to see if these cells are responsible for the alteration in surfactant lipids found in patients with ILD. Finally, we will test to see if there are growth factors for type II cells in lavages from patients or animals with interstitial lung disease. The proposed studies will focus on the role of parenchymal lung cells in the development of interstitial lung disease.

肺泡II型细胞是特化的上皮细胞， 肺泡合成和分泌肺表面活性物质 材料 II型细胞被认为是干细胞， 上皮细胞和II型细胞的增殖对于 肺泡损伤后气体交换单位的恢复。 的 假设，这一项目的基础上，是为功能 对于我们最终理解 间质性肺病。 此外，我们假设， II型细胞的功能状态可以部分地由以下确定： 分析灌洗液中表面活性剂的单个组分。 我们 计划分析34 kd载脂蛋白（载脂蛋白A）和 特发性肺动脉高压患者灌洗液中磷脂的变化 纤维化和对照以及用博来霉素处理的动物。 我们将开发维持人类和大鼠II型的方法， 在原代培养中处于分化状态的细胞。 这一目标非常 重要的是发现不同的细胞调节 表面活性剂组分。 具体来说，我们想确定 34kd脱辅基蛋白、饱和磷脂酰胆碱，和 磷脂酰甘油在II型细胞中独立调节 从成年动物。 我们计划开发分离 增生的II型细胞，看看这些细胞是否负责 ILD患者中发现的表面活性物质脂质的改变。 最后，我们将测试，看看是否有生长因子为II型 间质性肺的患者或动物的灌洗液中的细胞 疾病 拟议的研究将侧重于 肺实质细胞在肺间质发育中的作用 疾病