TYPE II CELLS AND INTERSTITIAL LUNG DISEASE

II 型细胞和间质性肺疾病

• 批准号: 3921142
• 负责人: ROBERT J MASON
• 金额: --
• 依托单位: NATIONAL JEWISH HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3921142
• 关键词: allergic pneumonitis; apolipoproteins; bleomycin; blood lipoprotein biosynthesis; cell growth regulation; cellular pathology; diagnostic respiratory lavage; disease /disorder model; extracellular matrix; fibronectins; human subject; hyperplasia; inflammation; laboratory rat; lipid biosynthesis; lung alveolus; mitogens; phospholipids; pulmonary surfactants; respiratory epithelium; silicates; surface antigens; tissue /cell culture

Alveolar type II cells are specialized epithelial cells that line the alveolus and synthesize and secrete pulmonary surface active material. Type II cells are thought to be the stem cell for epithelium and proliferation of type II cells is critical for the restoration of gas exchange units after alveolar injury. The hypothesis, on which this project is based, is that for the functions of type II cells are important in our ultimate understanding of interstitial lung disease. In addition, we hypothesize that the functional state of type II cells can be determined in part by analysis of individual components of surfactant in lavage. We plan to analyze the 34 kd apoprotein (apoprotein A) and the phospholipids in lavage from patients with idiopathic pulmonary fibrosis and controls and from animals treated with bleomycin. We will develop methods of maintaining human and rat type II cells in a differentiated state in primary culture. This aim is very important for discovering the cellular regulation of different components of surfactant. Specifically, we want to determine if the 34 kd apoprotein, saturated phosphatidylcholine, and phosphatidylglycerol are independently regulated in type II cells from adult animals. We plan to develop methods for isolating hyperplastic type II cells to see if these cells are responsible for the alteration in surfactant lipids found in patients with ILD. Finally, we will test to see if there are growth factors for type II cells in lavages from patients or animals with interstitial lung disease. The proposed studies will focus on the role of parenchymal lung cells in the development of interstitial lung disease.

肺泡II型细胞是一种特殊的上皮细胞，排列在 肺泡和合成分泌肺表面活性 材料。II型细胞被认为是 上皮细胞和II型细胞的增殖对 肺泡损伤后气体交换单元的恢复。这个 本项目所基于的假设是关于功能的假设 对于我们最终理解第二类细胞 间质性肺病。此外，我们假设 II型细胞的功能状态可以通过以下方式部分确定 灌洗液中表面活性物质各组分的分析。我们 计划分析34kd的载脂蛋白(载脂蛋白A)和 特发性肺病患者灌洗液中的磷脂 纤维化和对照，以及博莱霉素治疗的动物。 我们将开发维持人类和大鼠II型的方法 在原代培养中处于分化状态的细胞。这个目标是非常 对于发现不同的细胞调节很重要 表面活性剂的组成。具体地说，我们想确定 34kd的载脂蛋白、饱和磷脂酰胆碱和 磷脂酰甘油在II型细胞中独立调节 从成年动物身上。我们计划开发分离的方法 以确定这些细胞是否是 ILD患者表面活性物质脂的变化。 最后，我们将进行测试，以确定是否存在第二类增长因素 间质性肺病患者或动物灌洗液中的细胞 疾病。拟议的研究将重点放在 肺实质细胞在间质性肺发育中的作用 疾病。