BEHAVIOR OF BACTERIA--BIOCHEMISTRY AND GENETICS

细菌的行为——生物化学和遗传学

• 批准号: 3124447
• 负责人: JULIUS ADLER
• 金额: $ 14.83万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-12-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124447
• 关键词: Escherichia coli; bacterial genetics; bacterial proteins; biochemistry; cell growth regulation; cell motility; chemoreceptors; chemotaxis; electron microscopy; flagellum; genetic manipulation; membrane potentials

The objective of this project is to discover the mechanisms that bacteria use for getting attracted or repelled by chemicals (chemotaxis): 1) How are the chemicals sensed? We now know that there are chemoreceptors for this function. 2) How do bacterial flagella work? We now know that they function by rotaating, like a propellor, and they can rotate both clockwise and counterclockwise. 3) How is the sensed information transmitted to the flagella to tell them in which direction to rotate, in order to bring the bacterium toward the attractants or away from the repellents? We discovered the methylation of a set of membrane proteins, the so-called methyl-accepting chemotaxis proteins (MCPs), that serve to communicate between the chemoreceptors and the flagella. These proteins will be further studied during the coming year.

这个项目的目标是发现细菌使用的机制 被化学物质吸引或排斥(趋化性)：1)身体状况如何 感觉到化学物质了吗？我们现在知道，这一功能存在化学感受器。 2)细菌鞭毛是如何工作的？我们现在知道它们的功能是轮生的， 就像螺旋桨一样，它们可以顺时针和逆时针旋转。3) 感受到的信息是如何传递给鞭毛的，告诉它们 旋转方向，以便将细菌带向引诱剂或 远离驱虫剂？ 我们发现了一系列膜蛋白的甲基化，即所谓的 甲基接受趋化蛋白(MCP)，用于在 化学感受器和鞭毛。这些蛋白质将被进一步研究。 在新的一年里。