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Cold Spring Harbor Laboratory ADVANCED BACTERIAL GENETICS COURSE

冷泉港实验室高级细菌遗传学课程

基本信息

批准号：
0343656
负责人：
Terri Grodzicker
金额：
$ 37.81万
依托单位：
Cold Spring Harbor Laboratory
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2004
资助国家：
美国
起止时间：
2004-07-15 至 2009-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0343656&HistoricalAwards=false
关键词：
Cold Spring Harbor Laboratory ADVANCED

项目摘要

The Cold Spring Harbor Laboratory proposes to continue its highly successful summer course in Advanced Bacterial Genetics. This course has its roots in the original Bacteriophage Course initiated by Dr. Max Delbruck in 1945. This three week course prepares the participant to enter directly into research that makes use of advanced techniques and concepts in molecular genetics and microbial bioinformatics. Experiments providing a solid grounding in classical genetic analysis support training in contemporary uses of transposon mutagenesis, gene fusion technology, chromosomal mapping, deletion mapping and complementation analysis, second-site suppressor analysis and whole genome analysis. Experiments also introduce the student to new molecular techniques of gene cloning, new advances in site-directed mutagenesis, DNA microarray analysis and polymerase chain reaction as applied to diverse problems in bacterial and bacteriophage genetics, "mapping" genes by sequencing from transposon insertion ends and genome sequence searching, and the use of transcriptional, translational and cellular localization reporters along with indicator media and deconvolution microscopy to identify mutants. This course, which is unique in providing instruction in both genetics and molecular biology, gives participants the wherewithal required to perform analogous experiments in their home laboratories.

冷泉港实验室建议继续其在先进的细菌遗传学非常成功的夏季课程。本课程起源于1945年由Max Delbruck博士发起的原始噬菌体课程。这个为期三周的课程准备参与者直接进入研究，利用先进的技术和概念，在分子遗传学和微生物生物信息学。实验提供了坚实的基础，在经典的遗传分析支持培训在当代使用转座子诱变，基因融合技术，染色体定位，缺失定位和互补分析，第二个位点抑制分析和全基因组分析。实验还向学生介绍基因克隆的新分子技术、定点突变的新进展、应用于细菌和噬菌体遗传学中各种问题的DNA微阵列分析和聚合酶链反应、通过转座子插入测序“绘制”基因和基因组序列搜索，以及转录、翻译和细胞定位报告物沿着指示培养基和去卷积显微镜以鉴定突变体。这门课程在遗传学和分子生物学方面都是独一无二的，它为参与者提供了在家庭实验室进行类似实验所需的资金。