CSHL Advanced Bacterial Genetics Course

CSHL高级细菌遗传学课程

• 批准号: 1346974
• 负责人: Terri Grodzicker
• 金额: $ 25.5万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-15 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1346974&HistoricalAwards=false
• 关键词: CSHL; Advanced; Bacterial; Genetics; Course

Intellectual Merit: Cold Spring Harbor Laboratory's three-week summer course in Advanced Bacterial Genetics prepares participants to enter directly into research employing advanced techniques and concepts in molecular genetics. Students gain hands-on training in the theoretical knowledge and practical skills needed to analyze and manipulate bacterial genes and genomes both in vivo and in vitro. Experiments provide a solid grounding in classical genetic analysis, while supporting training in contemporary uses of transposon mutagenesis, gene fusion and reporter technology, complementation analysis, suppressor analysis, and gene transfer by conjugation, transduction and transformation. Experiments also introduce students to new molecular techniques of genetic engineering that can be applied to diverse problems in bacterial and bacteriophage genetics, such as bacteriophage recombination functions ("recombineering"), gene cloning using site-specific recombination, and advances in site-directed and linker-scanning mutagenesis. In addition, students receive bioinformatics training that is necessary for modern molecular genetics including PCR and oligonucleotide design and DNA sequence analysis. The course is taught by instructors, who are chosen based on their expertise and contributions to the field of bacterial genetics. The instructors invite guest lecturers to give up-to-the-minute reports on current research on diverse topics, while emphasizing the genetic methodologies they used to study these topics. A hallmark of the course is the free exchange of ideas and discussion between participants, instructors, and lecturers. Course participants are chosen from a pool of highly qualified applicants ranging from graduate students to senior investigators and coming from diverse research backgrounds, including biology, chemistry, engineering, physics, and mathematics.Broader Impacts: The course provides a collaborative environment for intense training in modern microbial genetics. The course trains an ethnically, racially and scientifically diverse group, stimulates interaction among all participants, and provides a route for productive research mentorship and engagement.

智力优势：冷泉港实验室为期三周的高级细菌遗传学夏季课程为参与者直接进入采用分子遗传学先进技术和概念的研究做好准备。学生获得在体内和体外分析和操纵细菌基因和基因组所需的理论知识和实践技能的实践培训。实验提供了一个坚实的基础，在经典的遗传分析，同时支持在当代使用转座子诱变，基因融合和报告技术，互补分析，抑制基因分析，并通过接合，转导和转化基因转移的培训。实验还向学生介绍基因工程的新分子技术，这些技术可以应用于细菌和噬菌体遗传学中的各种问题，例如噬菌体重组功能（“重组工程”），使用位点特异性重组的基因克隆，以及定点和接头扫描诱变的进展。此外，学生还将接受现代分子遗传学所必需的生物信息学培训，包括PCR和寡核苷酸设计以及DNA序列分析。该课程由教师授课，他们是根据他们的专业知识和对细菌遗传学领域的贡献而选择的。讲师邀请客座讲师就不同主题的当前研究给出最新的报告，同时强调他们用于研究这些主题的遗传方法。该课程的一个特点是参与者，教师和讲师之间的思想和讨论的自由交流。课程参与者是从高素质的申请者中挑选出来的，从研究生到高级研究人员，来自不同的研究背景，包括生物学，化学，工程学，物理学和数学。更广泛的影响：该课程为现代微生物遗传学的强化培训提供了一个合作环境。该课程培养了一个民族，种族和科学多样化的群体，刺激所有参与者之间的互动，并为富有成效的研究指导和参与提供了一条途径。