CSHL Advanced Bacterial Genetics Course

智力价值：冷泉港实验室为期三周的高级细菌遗传学暑期课程，为参与者利用分子遗传学中的先进技术和概念直接进入研究做准备。学生将获得在体内和体外分析和操作细菌基因和基因组所需的理论知识和实践技能方面的实践培训。实验为经典遗传分析奠定了坚实的基础，同时支持了转座子突变、基因融合和报告技术、互补分析、抑制子分析以及通过接合、转导和转化进行基因转移的当代应用方面的培训。实验还向学生介绍了可应用于细菌和噬菌体遗传学中各种问题的新的基因工程分子技术，如噬菌体重组功能(重组工程)，使用定点重组的基因克隆，以及定点定向和接头扫描突变的进展。此外，学生还接受现代分子遗传学所必需的生物信息学培训，包括聚合酶链式反应和寡核苷酸设计以及DNA序列分析。这门课程由讲师讲授，讲师是根据他们在细菌遗传学领域的专业知识和贡献来选择的。讲师邀请客座讲师提供关于当前不同主题研究的最新报告，同时强调他们用于研究这些主题的遗传方法论。该课程的一个特点是学员、讲师和讲师之间可以自由交流思想和进行讨论。课程参与者是从从研究生到高级研究人员的高素质申请者中挑选出来的，他们来自不同的研究背景，包括生物、化学、工程、物理和数学。广泛的影响：该课程为现代微生物遗传学的强化培训提供了一个合作的环境。该课程培训了一个种族、种族和科学多元化的群体，促进了所有参与者之间的互动，并为富有成效的研究指导和参与提供了一条途径。

Intellectual Merit: Cold Spring Harbor Laboratory's three-week summer course in Advanced Bacterial Genetics prepares participants to enter directly into research employing advanced techniques and concepts in molecular genetics. Students gain hands-on training in the theoretical knowledge and practical skills needed to analyze and manipulate bacterial genes and genomes both in vivo and in vitro. Experiments provide a solid grounding in classical genetic analysis, while supporting training in contemporary uses of transposon mutagenesis, gene fusion and reporter technology, complementation analysis, suppressor analysis, and gene transfer by conjugation, transduction and transformation. Experiments also introduce students to new molecular techniques of genetic engineering that can be applied to diverse problems in bacterial and bacteriophage genetics, such as bacteriophage recombination functions ("recombineering"), gene cloning using site-specific recombination, and advances in site-directed and linker-scanning mutagenesis. In addition, students receive bioinformatics training that is necessary for modern molecular genetics including PCR and oligonucleotide design and DNA sequence analysis. The course is taught by instructors, who are chosen based on their expertise and contributions to the field of bacterial genetics. The instructors invite guest lecturers to give up-to-the-minute reports on current research on diverse topics, while emphasizing the genetic methodologies they used to study these topics. A hallmark of the course is the free exchange of ideas and discussion between participants, instructors, and lecturers. Course participants are chosen from a pool of highly qualified applicants ranging from graduate students to senior investigators and coming from diverse research backgrounds, including biology, chemistry, engineering, physics, and mathematics.Broader Impacts: The course provides a collaborative environment for intense training in modern microbial genetics. The course trains an ethnically, racially and scientifically diverse group, stimulates interaction among all participants, and provides a route for productive research mentorship and engagement.