Utility of LOV-based Fluorescent Reporters To Monitor the Real-Time Secretion and Localisation of Bacterial Effector Proteins

点击翻译按钮获取中文摘要

The project aims to develop and test a novel fluorescent marker that we have been working on. This is called LOV, which stands for 'Light, Oxygen, Voltage'. We work on a form of LOV which emits a strong green fluorescence upon blue light excitation. We believe that LOV might act as an alternative to green fluorescent protein (GFP), used extensively in biology for many applications including the study of protein folding, protein transport, RNA dynamics and responses to infection. In this project we will test to see if LOV can be used to 'tag' proteins that are made by bacteria and then injected into host cells by a dedicated secretion system. The project is strengthened by using expertise from 3 different lead scientists: Dr Chrisitie is a biochemist who has led research on the application of LOV in novel areas of biology, Dr Roe is a microbiologist working on gene regulation and bacterial-host cell interactions and Dr Enninga, a named collaborator, is a specialist in imaging bacterial proteins.