A HEPATITIS VIRUS INFECTED GROUND SQUIRREL COLONY

感染肝炎病毒的地松鼠群体

• 批准号: 3130276
• 负责人: WILLIAM S ROBINSON
• 金额: $ 19.81万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-01-01 至 1987-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3130276
• 关键词: animal viral hepatitis; antiviral agents; chemical carcinogen; duck hepatitis B virus; genetic manipulation; hepatitis B virus group; hepatitis virus; hepatocellular carcinoma; nucleic acid sequence; tissue /cell culture; virus DNA; virus genetics; virus infection mechanism; virus replication

Three years ago we discovered a hepatitis virus in Beechey ground squirrels (GSHV) with ultrastructural, antigenic, molecular and biological properties similar to those of hepatitis B virus (HBV) of man and woodchuck hepatitis virus (WHV). In the past 3 years we have studied the host range of GSHV, its antigenic and polypeptide structure, its DNA structure and the viral DNA forms in infected liver. Little or no hepatitis and no hepatomas have been observed in chronically GSHV infected ground squirrels in contrast to moderately severe hepatitis and hepatomas frequently observed in WHV infected woodchucks and HBV infected humans. We propose to investigate the basis for the difference in incidence and severity of liver disease in the different virus-host systems. We will investigate the infectivity and pathogenicity of GSHV for woodchucks and WHV for ground squirrels to attempt to determine whether a difference in pathogenicity of the two virus, or a difference in response of the two hosts accounts for the observed difference in disease incidence. We will examine the viral DNA forms in GSHV infected ground squirrel liver and determine whether viral DNA becomes integrated in cellular DNA, and compare these findings with results in woodchuck and human systems. We will study the effects of environmental conditions and aflatoxin B1 on the pathogenic effects of GSHV in ground squirrels. We will study replication of GSHV DNA in infected liver and in tissue culture cells if they can be successfully infected. Finally, we will study the effect of antivirals on the high titer persistent infection seen in GSHV.

三年前我们在比奇地松鼠身上发现了肝炎病毒