DUCK HEPATITIS B VIRUS: INFECTION AND DISEASE

鸭乙型肝炎病毒：感染和疾病

• 批准号: 3172235
• 负责人: WILLIAM S ROBINSON
• 金额: $ 12.99万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-05-01 至 1988-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3172235
• 关键词: disease /disorder model; duck hepatitis B virus; embryo /fetus tissue /cell culture; genetic manipulation; hepatitis B antigens; hepatocellular carcinoma; monoclonal antibody; neoplasm /cancer genetics; virus DNA; virus genetics; virus infection mechanism; virus replication

We propose to continue our studies of duck hepatitis B virus (DHBV) infection of domestic ducks as an animal model of hepatitis B virus, which is associated with hepatitis, hepatomas, and immune complex disease in man. DHBV is present in 5-10% of the Peking ducks of many domestic flocks in the U.S. We plan to investigate the long-term course of DHBV-infection and document the associated disease. Hepatomas have been observed in areas of China where DHBV is endemic. We will study duck hepatomas from China and any that might appear in our flock for the presence of DHBV. Tumors will be analyzed for viral DNA by Southern blotting and integrated viral DNA will be cloned for further characterization. We will continue our analysis of DHBV surface and core antigen polypeptides, and will produce monoclonal as well as polyvalent antibodies to them to provide sensitive serological tests. We will study the appearance and relationship of different viral DNA forms during the course of infection in embryonated eggs, and attempt to grow virus in culture of duck embryo cells. Finally we will study the host range of DHBV by injection of the virus into different varieties of ducks, other species of fowl, and non-avian species. This virus-host system may offer certain advantages over other hepadna virus-host systems. Among these is the ease with which ducks and embryonated duck eggs can be obtained and experimentally manipulated, the ease with which embryos can be infected, the ease with which embryonic tissues can be cultured in vitro, the apparent difference in disease manifestations in different hosts (acute hepatitis in Khaki Campbell and not in Peking ducklings) and the possible association of the virus and hepatomas in ducks in China. These advantages justify the further development of the duck virus system in order to understand the mechanism of virus replication and the pathogenesis of diseases associated with infection.

我们建议继续我们对鸭乙型肝炎病毒(DHBV)的研究 感染家鸭作为乙肝病毒的动物模型 与肝炎、肝癌和免疫复合体疾病有关 天哪。在许多家养鸭群中，有5%-10%的北京鸭体内存在D乙肝病毒 在美国，我们计划调查D乙肝病毒感染的长期病程 并记录相关疾病。在一些地区观察到了肝癌 中国是登革热的地方性流行区。我们将从中国那里学习鸭肝癌 以及任何可能出现在我们群体中的D乙肝病毒。肿瘤 将通过Southern blotting和整合病毒进行病毒DNA分析 DNA将被克隆以进行进一步的鉴定。我们将继续我们的 分析DHBV表面和核心抗原多肽，并将产生 为它们提供敏感的单抗以及多价抗体 血清学测试。我们将研究…的出现和关系 胚胎感染过程中病毒DNA的不同形态 鸡蛋，并尝试在鸭胚胎细胞培养中培养病毒。终于 我们将通过将病毒注射到宿主体内来研究DHBV的宿主范围 不同品种的鸭子、其他种类的家禽和非禽类 物种。与其他病毒主机系统相比，这种病毒主机系统可能具有一定的优势 赫帕德纳病毒-宿主系统。其中之一是鸭子和鸭子很容易 可以获得胚胎鸭蛋并进行实验操作， 胚胎被感染的容易，胚胎感染的容易 组织可以在体外培养，疾病的明显区别 不同宿主的表现(卡其坎贝尔和卡其坎贝尔的急性肝炎 不是在北京雏鸭身上)，以及病毒与 中国鸭患肝癌。这些优势进一步证明了 为了解鸭病毒的致病机理而建立的鸭病毒系统 病毒复制和相关疾病的发病机制 感染。