DUCK HEPATITIS B VIRUS: INFECTION AND DISEASE

鸭乙型肝炎病毒：感染和疾病

• 批准号: 3172234
• 负责人: WILLIAM S ROBINSON
• 金额: $ 11.68万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-05-01 至 1988-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3172234
• 关键词: disease /disorder model; duck hepatitis B virus; embryo /fetus tissue /cell culture; genetic manipulation; hepatitis B antigens; hepatocellular carcinoma; monoclonal antibody; neoplasm /cancer genetics; virus DNA; virus genetics; virus infection mechanism; virus replication

We propose to continue our studies of duck hepatitis B virus (DHBV) infection of domestic ducks as an animal model of hepatitis B virus, which is associated with hepatitis, hepatomas, and immune complex disease in man. DHBV is present in 5-10% of the Peking ducks of many domestic flocks in the U.S. We plan to investigate the long-term course of DHBV-infection and document the associated disease. Hepatomas have been observed in areas of China where DHBV is endemic. We will study duck hepatomas from China and any that might appear in our flock for the presence of DHBV. Tumors will be analyzed for viral DNA by Southern blotting and integrated viral DNA will be cloned for further characterization. We will continue our analysis of DHBV surface and core antigen polypeptides, and will produce monoclonal as well as polyvalent antibodies to them to provide sensitive serological tests. We will study the appearance and relationship of different viral DNA forms during the course of infection in embryonated eggs, and attempt to grow virus in culture of duck embryo cells. Finally we will study the host range of DHBV by injection of the virus into different varieties of ducks, other species of fowl, and non-avian species. This virus-host system may offer certain advantages over other hepadna virus-host systems. Among these is the ease with which ducks and embryonated duck eggs can be obtained and experimentally manipulated, the ease with which embryos can be infected, the ease with which embryonic tissues can be cultured in vitro, the apparent difference in disease manifestations in different hosts (acute hepatitis in Khaki Campbell and not in Peking ducklings) and the possible association of the virus and hepatomas in ducks in China. These advantages justify the further development of the duck virus system in order to understand the mechanism of virus replication and the pathogenesis of diseases associated with infection.

我们建议继续对鸭B肝炎病毒进行研究 感染家鸭作为B型肝炎病毒的动物模型， 与肝炎、肝癌和免疫复合物疾病有关， 伙计 鸭乙型肝炎病毒存在于5-10%的北京鸭的许多国内羊群 在美国，我们计划研究DHBV感染的长期过程 并记录相关疾病。 在一些地区观察到了肝癌 在DHBV流行的中国。 我们将研究来自中国的鸭肝癌 以及任何可能出现在我们人群中的人 肿瘤 将通过Southern印迹分析病毒DNA，并整合病毒DNA。 将克隆DNA用于进一步表征。 我们将继续 分析DHBV表面和核心抗原多肽，并将产生 针对它们的单克隆以及多价抗体以提供敏感的 血清学测试 我们将研究的外观和关系， 不同的病毒DNA形式在感染过程中的胚胎 鸡蛋，并尝试在鸭胚细胞培养中培养病毒。 最后 我们将通过将病毒注射到 不同品种的鸭子，其他种类的家禽，和非鸟类 物种 这种病毒宿主系统可能比其他系统具有某些优势。 嗜肝DNA病毒-宿主系统。 这其中，有一个很简单的道理， 可以获得含胚鸭蛋并进行实验操作， 胚胎感染的容易程度， 组织可以在体外培养，疾病差异明显 不同宿主的表现（卡其坎贝尔和 北京雏鸭不存在），以及病毒与 中国鸭肝癌的研究进展 这些优点证明了进一步 发展鸭病毒系统，以了解机制 病毒复制和与之相关的疾病的发病机制 感染