X-RAY STRUCTURE OF AFRICAN TRYPANOSOME SURFACE ANTIGENS
X-RAY STRUCTURE OF AFRICAN TRYPANOSOME SURFACE ANTIGENS
批准号:
2061465
负责人:
DON C WILEY
金额:
$10.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-09-01 至 1995-07-31
关键词:
中文摘要
三维结构的一系列“可变面”
英文摘要
The three-dimensional structure of a series of "variable surface
glycoprotein" (VSG) antigens from the membrane of the unicellular
parasite, Trypanosoma brucei, are being determined by high resolution X-
ray crystallography. The African trypanosome evades the host immune
response by sequentially expressing antigenically distinct VSGs, which
form a protective coat on its surface. Just as the host responds to one
foreign antigenic challenge, the trypanosome expresses a new
antigenically distinct VSG, escaping neutralization by the host. Our goal
is to understand the structure of this remarkable series of surface
glycoproteins and their role in antigenic variation.
African trypanosomes, which are spread by the tsetse fly, cause various
forms of animal and human trypanosomiasis (sleeping sickness). These
studies aim to describe the structure of the VSG protein and
carbohydrate, the location and nature of the antigenic determinants, and
should provide a molecular model of the VSG, which can be used for the
rational design of drugs and/or vaccines.
Currently, the structures of the variable N-Terminal domain of MITat 1.2
and ILTat 1.25 VSGs are being determined to 2.9 and 2.5A resolution
respectively from crystals of the purified glycoproteins. Future studies
on complexes with monoclonal antibody Fab fragments and on electron
microscopic image analysis of the VSG coat are proposed.
期刊论文(3)
专著(0)
科研奖励(0)
会议论文
Crystallization and preliminary X-ray analysis of an intact soluble-form variant surface glycoprotein from the African trypanosome, Trypanosoma brucei.
来自非洲锥虫布氏锥虫的完整可溶形式变异表面糖蛋白的结晶和初步 X 射线分析。
DOI:
10.1016/0022-2836(91)90254-4
发表时间:
1991
期刊:
Journal of molecular biology
影响因子:
5.6
作者:
[Down,JA, Garman,SC, Gurnett,AM, Turner,MJ, Wiley,DC]
通讯作者:
Wiley,DC
Crystallization of amino-terminal domains and domain fragments of variant surface glycoproteins from Trypanosoma brucei brucei.
布氏锥虫表面糖蛋白变异体的氨基末端结构域和结构域片段的结晶。
DOI:
--
发表时间:
1988
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
[Metcalf,P, Down,JA, Turner,MJ, Wiley,DC]
通讯作者:
Wiley,DC
MACCHESS CONSORTIUM FOR SYNCHROTRON RADIATION IN MOLEC MED: TCR, MHC
-
批准号:6667831
-
项目类别:
-
资助金额:$14.27万
-
财政年份:2002
-
负责人:DON C WILEY
-
依托单位:
INHIBITORS OF GP41 MEDIATED HIV1 MEMBRANE FUSION
-
批准号:6631266
-
项目类别:
-
资助金额:$10.66万
-
财政年份:2002
-
负责人:DON C WILEY
-
依托单位:
INHIBITORS OF GP41 MEDIATED HIV1 MEMBRANE FUSION
-
批准号:6468920
-
项目类别:
-
资助金额:$10.66万
-
财政年份:2001
-
负责人:DON C WILEY
-
依托单位:
MACCHESS CONSORTIUM FOR SYNCHROTRON RADIATION IN MOLEC MED: TCR, MHC
-
批准号:6491154
-
项目类别:
-
资助金额:$14.27万
-
财政年份:2001
-
负责人:DON C WILEY
-
依托单位:
MACCHESS CONSORTIUM FOR SYNCHROTRON RADIATION IN MOLEC MED: TCR, MHC
-
批准号:6339166
-
项目类别:
-
资助金额:$2.23万
-
财政年份:2000
-
负责人:DON C WILEY
-
依托单位:
INHIBITORS OF GP41 MEDIATED HIV1 MEMBRANE FUSION
-
批准号:6336537
-
项目类别:
-
资助金额:$14.35万
-
财政年份:2000
-
负责人:DON C WILEY
-
依托单位:
MHC CLASS II STRUCTURE STUDIES
-
批准号:6201295
-
项目类别:
-
资助金额:$17.41万
-
财政年份:1999
-
负责人:DON C WILEY
-
依托单位:
INHIBITORS OF GP41 MEDIATED HIV1 MEMBRANE FUSION
-
批准号:6107542
-
项目类别:
-
资助金额:$14.35万
-
财政年份:1999
-
负责人:DON C WILEY
-
依托单位:
MACCHESS CONSORTIUM FOR SYNCHROTRON RADIATION IN MOLEC MED: TCR, MHC
-
批准号:6220526
-
项目类别:
-
资助金额:$2.23万
-
财政年份:1999
-
负责人:DON C WILEY
-
依托单位:
SUPERANTIGEN CLASS II MHC COMPLEX STRUCTURE AND FUNCTION
-
批准号:6108386
-
项目类别:
-
资助金额:$22.15万
-
财政年份:1998
-
负责人:DON C WILEY
-
依托单位:
MHC CLASS II STRUCTURE STUDIES
-
批准号:6100054
-
项目类别:
-
资助金额:$17.41万
-
财政年份:1998
-
负责人:DON C WILEY
-
依托单位:
MEMBRANE GLYCOPROTEINS FROM VIRUSES & FROM HUMAN CELLS
-
批准号:6281317
-
项目类别:
-
资助金额:$3.94万
-
财政年份:1998
-
负责人:DON C WILEY
-
依托单位:
INHIBITORS OF GP41 MEDIATED HIV-1 MEMBRANE FUSION
-
批准号:6271755
-
项目类别:
-
资助金额:$15.47万
-
财政年份:1998
-
负责人:DON C WILEY
-
依托单位:
MEMBRANE GLYCOPROTEINS FROM VIRUSES & FROM HUMAN CELLS
-
批准号:6120544
-
项目类别:
-
资助金额:$0.02万
-
财政年份:1998
-
负责人:DON C WILEY
-
依托单位:
CONFORMATIONS OF THE HIV1 ENVELOPE GLYCOPROTEIN
-
批准号:2555202
-
项目类别:
-
资助金额:$21.45万
-
财政年份:1997
-
负责人:DON C WILEY
-
依托单位:
MHC CLASS II STRUCTURE STUDIES
-
批准号:6235473
-
项目类别:
-
资助金额:$12.69万
-
财政年份:1997
-
负责人:DON C WILEY
-
依托单位:
CONFORMATIONS OF THE HIV1 ENVELOPE GLYCOPROTEIN
-
批准号:2673191
-
项目类别:
-
资助金额:$21.45万
-
财政年份:1997
-
负责人:DON C WILEY
-
依托单位:
SUPERANTIGEN CLASS II MHC COMPLEX STRUCTURE AND FUNCTION
-
批准号:6240938
-
项目类别:
-
资助金额:$21.39万
-
财政年份:1997
-
负责人:DON C WILEY
-
依托单位:
MEMBRANE GLYCOPROTEINS FROM VIRUSES & FROM HUMAN CELLS: HIV
-
批准号:6251667
-
项目类别:
-
资助金额:$1.12万
-
财政年份:1997
-
负责人:DON C WILEY
-
依托单位:
MHC LINKED SUSCEPTIBILITY TO AUTOIMMUNITY--STRUCTURE
-
批准号:2672734
-
项目类别:
-
资助金额:$87.04万
-
财政年份:1996
-
负责人:DON C WILEY
-
依托单位:
海外基金