MOLECULAR ANALYSIS OF HEPATITIS B VIRUS

拟议的研究可以分为两个主要方面。 第一 关注对B型肝炎病毒（HBV）生命周期的研究。 的目的 这项工作是对病毒的全面了解，以及它是如何 在肝细胞中进行自我复制 它包括（1）a HBV基因（表面（HBsAg）、核心（HBcAg））的综合分析 抗原，DNA聚合酶？，和750 b RNA）和它们在体内的表达， 体外 另外，我们将确定“e”抗原的性质。 (2)病毒的假定复制形式分析和对病毒的控制 病毒的复制。 (3)22 nm的分子表征 HBsAg颗粒。 (4)嗜肝性分子基础的研究 寻找一个假定的HBV受体。 （5）体外系统的开发 用于研究病毒的感染性和营养生长。 第二 主要方面涉及HBV在疾病中的作用的分析。 目的 这些研究的目的是阐明HBV诱导的免疫应答的分子基础。 直接检测HBV是否为原发性肝癌的病原体。 该建议包括：（1）研究各种病毒DNA形式， 感染者肝脏和血清中的病毒源性HBsAg颗粒 患者-各种形式的肝炎（自限性肝炎，暴发性肝炎， 肝炎、慢性肝炎、携带者）和原发性肝细胞癌 癌（HPC）。 (2)乙型肝炎病毒序列的结构和表达分析 整合在肝癌的基因组DNA中， 这种融合。 这可能揭示了一个基本的 本体论过程 (3)测试单个或成对的表达式是否 会导致细胞病变。 这些研究将有助于 我们对病毒和病毒性疾病的了解。 他们可能会照亮 世界上最流行的癌症的病因。 此外，他们将 有助于了解基因结构的基本分子细节， 调节哺乳动物细胞中的基因表达以及 宿主组织特异性的细节。

The proposed research can be divided into two main aspects. The first concerns studies on the hepatitis B virus (HBV) life cycle. The aim of this work is a comprehensive understanding of the virus and how it functions to replicate itself in the hepatic cell. It includes (1) a comprehensive analysis of HBV genes (surface (HBsAg), core (HBcAg) antigens, DNA polymerase?, and a 750b RNA) and their expression in vivo and in vitro. In addition, we will determine the nature of the "e" antigen. (2) Analysis of presumed replicative forms of the virus and the cotnrol of replication of the virus. (3) The molecular characterization of the 22nm HBsAg particle. (4) A study of the molecular basis of hepatotropism--a search for a putative HBV receptor. (5 Development of an in vitro system for studying infectivity and vegetative growth of the virus. The second major aspect concerns an analysis of the role of HBV in disease. The aim of these studies is to elucidate the molecular basis of HBV induced hepatitis and to directly test whether HBV os tje causative agent of PHC. The proposal includes (1) studies of the various viral DNA forms andof virus-derived HBsAg particles in the liver and serum of infected patients--various forms of hepatitis (self limited hepatitis, fulminant hepatitis, chronic hepatitis, carriers), and in primary hapatocellular carcinoma (HPC). (2) Analysis of structure and expression of HBV sequences integrated in genomic DNA of hapatomas and the biochemical consequences of this integration. This may reveal the molevular outline of a basic ontological process. (3) Tests whether the expression of single or pairs of virus genes will cause cellular lesions. These studies will contribute to our knowledge of viruses and viral diseases. They may illuminate the etiology of the world's most prevalent cancer. In addition they will contribute undersanding of basic molecular details of gene structure, and regulation of gene expression in mammalian cells as well as the molecular specifics of host-tissue specificity.