MOLECULAR ANALYSIS OF HEPATITIS B VIRUS

这项研究旨在阐明生命中尚未解决的特征， HBV周期，包括各种基因表达的调节 和HBV病毒颗粒的形成，并进一步研究 HBV相关的肿瘤发生。 需要解决的具体问题包括：1） 两种表面抗原的作用和可能的独立调节 启动子，大表面抗原的功能分析 产品，P45，核心启动子的表征，特别是在 与前核心和核心转录本的形成的关系，以及 聚合酶基因的可能形成。 我们还将调查 核心启动子与相邻pol III启动子的相互作用 指导从短链形成RNA。 2)进一步 HBV增强子的表征，包括精细定位和突变 分析、测定其对各种HBV启动子的活化， 反式作用（肝脏特异性）因子的阐明 增强子活性 3)核心结构功能的阐明 包括a）前核在E抗原形成中的作用， 包括e抗原结构的测定; B）抗原的作用， sAg/cAg复合结构的形成。 4)的作用 整合的HBV序列在HCC肿瘤发生中的作用。 这些研究涉及 表征约20个分离的肝炎特异性和肿瘤特异性 基因.

The proposed research aims to elucidate unresolved features in the life cycle of HBV including the regulation of expression of the various genes and the formation of the HBV viral particles, and to further investigate HBV-associated oncogenesis. The specific issues to be addressed include 1) the role and possible independent regulation of the two surface antigen promoters, an analysis of the function of the large surface antigen product, P45, characterization of the core promoter specifically in relation to the formation of the precore and core transcripts, and the possible formation of the polymerase gene. We will also investigate the interaction of the core promoter with a contiguous pol III promoter directing the formation of an RNA from the short strand. 2) Further characterization of the HBV enhancer including fine mapping and mutational analysis, determination of its activation of the various HBV promoters and elucidation of trans-acting (liver-specific) factor responsible for enhancer activity. 3) Elucidation of the function of core structures including a) the role of precore in the formation of the e antigen, including the determination of e antigen structures; b) the role of the precore in the formation of sAg/cAg compositive structures. 4) The role of integrated HBV sequences in HCC oncogenesis. These studies involve characterization of about 20 isolated hepatitis-specific and tumor-specific genes.