DIFFERENTIATION IN THE ENDOCRINE AND EXOCRINE PANCREAS

内分泌胰腺（胰岛素）和胰腺癌的单个基因产物的DNA探针 和潜在的胰高血糖素）和外分泌胰腺（几种消化酶 包括淀粉酶）已经通过mRNA的逆转录制备， 随后在E. coli X1776。 我们建议利用克隆的 结构基因序列，以探测组织和表达的 内分泌和外分泌特异性基因。 克隆的序列将用于 分离（通过在E. coli X1776）胰岛素和淀粉酶基因序列 大鼠核DNA 分离的基因将允许序列的研究 结构基因及其相邻DNA区域的组织。 的 克隆的结构基因也将用于研究它们表达的变化 在胚胎发育过程中， 致病状态

DNA probes for individual gene products of both the endocrine pancreas (insulin and potentially glucagon) and the exocrine pancreas (several digestive enzymes including amylase) have been prepared by reverse transcription of mRNA and subsequent cloning in E. coli X1776. We propose to utilize the cloned structural gene sequences to probe the organization and expression of the endocrine and exocrine specific genes. The cloned sequences will be used to isolate (by cloning in E. coli X1776) insulin and amylase gene sequences from rat nuclear DNA. The isolaed genes will permit the study of the sequence organization of the structural genes and their contiguous DNA regions. The cloned structural genes will also be used to study changes in their expression during embryonic development as well as alteration in expression accompanying pathogenic states.