EPIDEMIOLOGY OF ANTIBIOTIC RESISTANCE PLASMIDS

抗生素耐药性质粒的流行病学

• 批准号: 3135626
• 负责人: THOMAS FRANCIS O'BRIEN
• 金额: $ 12.5万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1986-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3135626
• 关键词: antibiotics; bacterial DNA; bacterial genetics; computer data analysis; developmental genetics; endonuclease; genetic mapping; nucleic acid sequence; plasmids

The specific aims of the project are to detect distinctive antibiotic resistance plasmids in bacterial isolates from one or more centers, trace their distribution and changes in distribution over time and relate this deployment to factors in their environment and alterations in their genetic structure. The methodology is to exploit a large existing base of data on quantitative antibiotic susceptibility test results from multiple centers, and to use existing computer programs which are able to detect distinctive antibiotypes produced by one or a small number of plasmids. Plasmids from representative isolates with the antibiotype are extracted and their structures compared by endonuclease restriction digestion analysis. When a correlation between plasmid structure and quantitative antibiotype is established, the epidemiology of the plasmid is traced and analyzed. Selected significant plasmids will be mapped to permit interpretation of structural or of functional variants. The project seeks to link the now advanced understanding of plasmid molecular structure with important issues of clinical antibiotic use through the complex epidemiology of antibiotic resistance plasmids, which connects the two subjects but for which there are now only fragmentary observations.

该项目的具体目标是检测独特的抗生素 来自一个或多个中心的细菌分离株中的耐药质粒，痕量 它们的分布和分布随时间的变化， 部署到他们的环境中的因素和改变他们的基因 结构 方法是利用现有的大量数据， 来自多个中心的定量抗生素敏感性测试结果， 并使用现有的计算机程序， 由一个或少数质粒产生的质粒型。 Plasma from 提取具有代表性的菌株， 通过内切酶限制性消化分析比较结构。 当一个 质粒结构与定量PCR类型之间的相关性是 建立了质粒的流行病学追踪和分析。 将对选定的重要质粒进行图谱分析，以解释 结构或功能变体。 该项目旨在将现在联系起来 深入了解质粒分子结构和重要问题 通过抗生素的复杂流行病学 耐药质粒，它连接两个主题，但其中有 现在只是零星的观察。