EPIDEMIOLOGY OF ANTIBIOTIC RESISTANCE PLASMIDS

抗生素耐药性质粒的流行病学

• 批准号: 3135625
• 负责人: THOMAS FRANCIS O'BRIEN
• 金额: $ 15.45万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-03-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3135625
• 关键词: Escherichia coli; Klebsiella; Salmonella; antibiotics; bacterial DNA; bacterial genetics; computer data analysis; developmental genetics; endonuclease; enteric bacteria; evolution; feces; genetic manipulation; genetic mapping; molecular cloning; nucleic acid probes; nucleic acid sequence; plasmids; transposon /insertion element

The long term objectives of the research are to improve understanding of the evolution and diversity of antibacterial resistance plasmids in globally dispersed populations of bacteria in order to improve understanding and control of the spread of antibacterial resistance genes. The specific aims are to determine and computer file EcoRI fragment size values for resistance plasmids selected by resistance phenotype or randomly from an extensive series of isolate collections, to detect in this data further copies of previously recognized stable plasmids and further delineate their distributions, to detect and delineate distribution of additional stable plasmids and estimate their diversity, to develop methods for detecting and analyzing lesser relatedness between plasmids, to survey resistance plasmid prevalence, diversity and identity in fecal E. coli from untreated and unhospitalized humans in different parts of the world, to adapt and apply to data on fragment sizes of may isolates on one plasmid available statistical programs to reconstruct the plasmid's phylogenetic tree, and to detect and establish fine detail of regions involved in recombinational events that are significant for the spread of antibacterial resistance genes in globally dispersed populations of bacteria.

这项研究的长期目标是改进

项目成果

Functional and structural map of pLST1000: a multiresistance plasmid widely distributed in Enterobacteriaceae.

pLST1000 的功能和结构图：广泛分布于肠杆菌科的多抗性质粒。

• DOI: 10.1016/0147-619x(88)90021-2
• 发表时间: 1988
• 期刊: Plasmid
• 影响因子: 2.6
• 作者: Hopkins,JD;O'Brien,TF;Syvanen,M
• 通讯作者: Syvanen,M

Plasmid diversity in Escherichia coli isolated from processed poultry and poultry processors.

从加工家禽和家禽加工商中分离出的大肠杆菌中的质粒多样性。

• DOI: 10.1016/0378-1135(93)90149-2
• 发表时间: 1993
• 期刊: Veterinary microbiology
• 影响因子: 3.3
• 作者: O'Brien,TF;DiGiorgio,J;Parsonnet,KC;Kass,EH;Hopkins,JD
• 通讯作者: Hopkins,JD

Gly-238-Ser substitution changes the substrate specificity of the SHV class A beta-lactamases.

Gly-238-Ser 取代改变了 SHV A 类 β-内酰胺酶的底物特异性。

• DOI: 10.1002/prot.340110106
• 发表时间: 1991
• 期刊: Proteins
• 影响因子: 2.9
• 作者: Lee,KY;Hopkins,JD;O'Brien,TF;Syvanen,M
• 通讯作者: Syvanen,M

Loss of OmpC porin in a strain of Salmonella typhimurium causes increased resistance to cephalosporins during therapy.

• DOI: 10.1093/infdis/156.5.751
• 发表时间: 1987-11
• 期刊: The Journal of infectious diseases
• 作者: A. Medeiros;T. O'Brien;E. Rosenberg;H. Nikaido