EPIDEMIOLOGY OF ANTIBIOTIC RESISTANCE PLASMIDS

抗生素耐药性质粒的流行病学

• 批准号: 3135624
• 负责人: THOMAS FRANCIS O'BRIEN
• 金额: $ 14.77万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-03-01 至 1992-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3135624
• 关键词: Escherichia coli; Klebsiella; Salmonella; antibiotics; bacterial DNA; bacterial genetics; computer data analysis; developmental genetics; endonuclease; enteric bacteria; evolution; feces; genetic manipulation; genetic mapping; molecular cloning; nucleic acid probes; nucleic acid sequence; plasmids; transposon /insertion element

The long term objectives of the research are to improve understanding of the evolution and diversity of antibacterial resistance plasmids in globally dispersed populations of bacteria in order to improve understanding and control of the spread of antibacterial resistance genes. The specific aims are to determine and computer file EcoRI fragment size values for resistance plasmids selected by resistance phenotype or randomly from an extensive series of isolate collections, to detect in this data further copies of previously recognized stable plasmids and further delineate their distributions, to detect and delineate distribution of additional stable plasmids and estimate their diversity, to develop methods for detecting and analyzing lesser relatedness between plasmids, to survey resistance plasmid prevalence, diversity and identity in fecal E. coli from untreated and unhospitalized humans in different parts of the world, to adapt and apply to data on fragment sizes of may isolates on one plasmid available statistical programs to reconstruct the plasmid's phylogenetic tree, and to detect and establish fine detail of regions involved in recombinational events that are significant for the spread of antibacterial resistance genes in globally dispersed populations of bacteria.

这项研究的长期目标是改进 对抗菌药物的进化和多样性的认识 全球分散的细菌种群中的耐药质粒 以便更好地了解和控制艾滋病的传播 抗菌耐药基因。 具体目标是确定和计算机文件EcoRI 由选择的抗性质粒的片段大小值 抗性表型或随机从广泛的一系列 隔离集合，以在此数据中检测 并进一步描述了它们之间的相互关系 分布，以检测和描绘附加的分布 稳定的质粒并估计它们的多样性，以开发方法 为了检测和分析质粒之间较小的关联性， [目的]调查沙门氏菌耐药质粒的流行率、多样性和特性 未经治疗和未住院的人的粪便大肠埃希菌 以适应和应用于世界不同地区的数据 同一质粒上可能分离株的片段大小统计 重建该质粒的系统发育树的程序，并 检测并确定涉及的区域的详细信息 对病毒传播具有重要意义的重组事件 分布在全球各地的日本血吸虫人群中的抗菌素耐药性基因 细菌。