CHARACTERIZATION/TICK ANTIGENS ELICITING HOST IMMUNITY

在这项拨款提案中，研究提出，以确定哪些蜱 （Amblyomma americanum）唾液腺抗原是导致 诱导和激发宿主免疫抗性。 各种唾液 将从活蜱中采集腺源性物质，并测定 通过体外和体内技术测定抗原性。 结果从这些 研究将导致分离和表征的 耐药相关抗原的各种色谱和 电泳技术 这些研究将阐明 抗原产生和释放。 一旦关键的蜱抗原进入 另一方面，这些物质将被测试其免疫宿主的能力， 对抗蜱虫 由于我们已经有了纯的抗蜱抗体（7SIGG1）， 一方面，使用这种重要的试剂作为探针将有助于我们 寻找关键的蜱抗原 此外，我们计划利用小鼠作为 蜱宿主产生针对各种蜱的单克隆抗体 抗原 这些高度特异性抗体的产生会增加 我们的一系列探针能够从粗蜱中分离出关键抗原 萃取物 关键抗原在手， 测序，并最终将相应的基因插入到 细菌，可以实现大规模抗原生产， 用于牛的疫苗开发。 开发这些新的 技术是需要多年复杂工作的重大步骤，但 重要的第一步已经完成，即分离特定的 一种转移免疫抗性的抗体（7SIGG1），可能是单特异性的 关键的蜱虫抗原 拟议的研究涵盖以下学科： 昆虫学、寄生虫学、免疫学和分子生物学。 虽然 拟议的研究是复杂和复杂的，这些不同的领域， 研究是在我们的能力和新的信息的理论和 应该产生实际的重要性。

In this grant proposal, studies are presented to determine which tick (Amblyomma americanum) salivary gland antigens are responsible for the induction and elicitation of host immune resistance. Various salivary gland derived substances will be collected from live ticks and assayed for antigenicity by in vitro and in vivo techniques. The results from these studies will lead to the isolation and characterization of the resistance-associated antigens by various chromatographic and electrophoretic techniques. Such studies will elucidate the kinetics of antigen production and release. Once the crucial tick antigens are in hand, these substances will be tested for their ability to immunize hosts against ticks. Since we already have pure anti-tick antibody (7SIgG1) in hand, the use of this important reagent as a probe will facilitate our search for crucial tick antigens. In addition, we plan to utilize mice as tick hosts to generate monoclonal antibodies directed at various tick antigens. The production of these highly specific antibodies will increase our reportoire of probes able to isolate crucial antigens from crude tick extracts. With the crucial antigen(s) in hand for eventual amino acid sequencing, and eventual insertion of the corresponding genes into bacteria, it may be possible to achieve large scale antigen production for vaccine development for use in cattle. Exploitation of these newer technologies are big steps that require years of complex work, but the essential first step has been achieved, that is the isolation of specific antibody (7SIgG1) that transfers immune resistance and may be monospecific for a crucial tick antigen. The studies proposed span the disciplines of entomology, parasitology, immunology and molecular biology. Although the proposed studies are complex and sophisticated, these diverse areas of research are within our ability and new information of theoretical and practical importance should be generated.