STRUCTURAL AND BIOLOGICAL PROPERTIES OF IDIOTYPES

研究的目的是：(1)获取更多有关 免疫球蛋白的结构和体细胞变异的性质 对生殖系基因进行操作。对于独特型家族的抗体，尝试 将使序列与一个或多个 (2)更好地定义和量化抗独特型 表达原始抗原的内部图像，并使用原理 用于诱导抗病毒抗体；(3)研究序列变异 在已确定的独特型IgE抗体中比较其性质和 (4)与其他班级的差异程度； 启动对免疫球蛋白E调节的研究。将与项目1接洽 通过X射线结晶学，由R.Poljak博士对单克隆进行 我们提供的抗体，并通过氨基酸序列的测定 (从mRNA序列推断)。次要的白痴类型的序列，即 与主要的交叉反应独特型密切相关但不同， 应该为生殖系DNA序列的变异程度增加一个维度 这是可以调查的。我们将量化表达内部的抗-ID 抗原在几个系统中的成像并试图诱导抗病毒 伪狂犬病病毒抗体(可能具有保护性)和 巨细胞病毒。表达主要和次要免疫球蛋白的单抗 将对独特型进行测序，以确定 这个搞笑班。最后，敏感的定量技术对总和 将使用携带独特型IgE抗苯丙氨酸抗体来启动 免疫球蛋白E调节的研究。 这些研究应该增加我们对抗体机制的了解。 生物合成和调节。关于结构变化的信息 抗体与我们对抗体生物合成的理解有关。 某些抗独特型抗体中抗原内像的研究 可能导致使用这种抗体作为疫苗或作为调节剂 涉及细胞表面受体的生理过程。免疫球蛋白E的研究 及其调控与控制即刻反应有关。 过敏症，包括过敏。

The research objectives are: (1) to obtain more information on the structure of immunoglobulins and on the nature of somatic variations that operate on germline genes. For antibodies of an idiotypic family, attempts will be made to correlate sequences with the 3-D structure of one or more members of the family; (2) to better define and quantify antiidiotypes that express an internal image of the original antigen, and to use the principle for induction of antiviral antibodies; (3) to study sequence variations among IgE antibodies of defined idiotype and to compare the nature and extent of these variations with those occurring in other classes; (4) to initiate studies on the regulation of IgE. Project 1 will be approached through X-ray crystallography, carried out by Dr. R. Poljak on monoclonal antibodies we are supplying, and by determination of amino acid sequences (inferred from mRNA sequences). The sequences of minor idiotypes, that are closely related to but divergent from a major cross-reactive idiotype, should add a dimension to the degree of variation on germline DNA sequences that can be investigated. We will quantify anti-Id expressing an internal image of the antigen in several systems and attempt to induce antiviral antibodies (possibly protective) against pseudorabies virus and cytomegalovirus. IgE monoclonal antibodies that express major and minor idiotypes will be sequenced to ascertain the patterns of variability in this Ig class. Finally, sensitive quantitative techniques for total and idiotype-bearing IgE antiphenylarsonate antibodies will be used to initiate studies of IgE regulation. These studies should add to our knowledge of mechanisms of antibody biosynthesis and regulation. Information on structural variations in antibodies is relevant to our understanding of antibody biosynthesis. Studies on internal images of antigens in certain antiidiotypic antibodies may lead to the use of such antibodies as vaccines or as modulators of physiological processes involving cell surface receptors. Studies of IgE and its regulation are relevant to the control of reactions of immediate hypersensitivity, including allergies.