ROLE OF IDIOTYPES IN THE IMMUNE SYSTEM

拟议的项目涉及到分子结构和调控， 免疫系统 单个杂交瘤产物（HP），表达主要的 A系小鼠抗苯胂酸（抗Ar）抗体独特型 独特的独特型决定因素，没有发现其他惠普的测试，但存在于 A株免疫血清。 我们将研究一些这样的个体发育 决定因素，以获得额外的信息，以确定是否出现 特定的克隆型是由基因编程的。 在合作研究中，我们 将决定是否自身抗id抗体出现在早期免疫 对Ar半抗原的反应（如TNP的情况），并干扰斑块 阵 使用一个明确的独特型系统应有助于新的 了解这个监管系统的方法。 我们将尝试 确定调节性T细胞是否响应于私人独特型而形成， 决定因素;调节性T细胞是否在响应私人 独特型决定因素;即独特型调节是否仅限于 大白痴 实验将通过使用独特型共轭 同基因细胞的抗独特型抗体特异性的私人决定因素。 效应T细胞库的程度将通过确定 这种私人独特型决定因素是否存在于T细胞上 治疗迟发型超敏反应 据了解，白痴型特异性辅助T细胞 主要独特型的大量表达需要细胞。 我们将 确定这种辅助细胞是否也需要表达次要的 独特型 这解决了需要抗独特型抗体的一般问题。 帮助表达各种克隆型。 其他人关于环境影响的工作 抑制性T细胞对B细胞肿瘤分泌抗体的作用将通过以下方式延长： 使用带有独特型和抗独特型受体的T细胞。 将努力 为了结晶Fab片段，用于结晶学， 携带主要独特型的HP。 我们将提供杂交瘤用于 研究控制T细胞受体的基因，并试图获得 用于那些实验的连续T细胞系。

The projects proposed relate to molecular structures and regulation in the immune system. Individual hybridoma products (HP's), expressing the major idiotype of anti-phenylarsonate (anti-Ar) antibodies of strain A mice, bear unique idiotypic determinants, not found on other HP's tested but present in strain A immune serum. We will study the ontogeny of a number of such determinants to obtain additional information as to whether the appearance of particular clonotypes is genetically programmed. In collaborative research we will determine whether auto-anti-id antibodies appear during the early immune response to the Ar hapten (as is the case for TNP), and interfere with plaque formations. The use of a well-defined idiotypic system should facilitate new approaches toward understanding this regulatory system. We will attempt to determine whether regulatory T cells are formed in response to private idiotypic determinants; whether regulatory T cells are formed in response to private idiotypic determinants; i.e. whether idiotypic regulation is restricted to the major idiotypes. The experiments will be done by using idiotype-conjugated syngeneic cells for anti-idiotypic antibodies specific for private determinants. The extent of the effector T cell repertoire will be explored by determining whether such private idiotypic determinants are present on T cells responsible for delayed-type hypersensitivity. It is known that idiotype-specific helper T cells are required for substantial expression of the major idiotype. We will ascertain whether such helper cells are also needed for expression of a minor idiotype. This addresses the general question of the need for antiidiotypic help in the expression of various clonotypes. Work of others on the effects of suppressor T cells on secretion of antibody by B cell tumors will be extended by using T cells with idiotypic and antiidiotypic receptors. Efforts will be made to crystallize Fab fragments, for use in crystallography, of a number of anti-Ar HP's that carry the major idiotype. We will provide hybridomas to be used in a study of the genes controlling the T cell receptor and will attempt to obtain continuous T cell lines for use in those experiments.