STRUCTURAL AND BIOLOGICAL PROPERTIES OF IDIOTYPES

本研究的目的是：（1）获得更多的信息， 免疫球蛋白的结构和体细胞变异的性质， 对生殖细胞基因起作用。 对于独特型家族的抗体，尝试 将使序列与一个或多个 （2）更好地定义和量化抗独特型， 表达原始抗原的内部图像，并使用该原理 用于诱导抗病毒抗体;（3）研究序列变异 确定的独特型的IgE抗体之间，并比较性质， 这些变化与其他类别中发生的变化的程度;（4） 启动IgE调节的研究。 项目1将与 通过X射线晶体学，由R. Poljak on monoclonal 我们提供的抗体，并通过测定氨基酸序列 （从mRNA序列推断）。 次要独特型的序列， 与主要交叉反应独特型密切相关但不同， 应该增加一个维度的程度变化的生殖系DNA序列 可以调查的。 我们将定量表达内部 在几个系统中的抗原图像，并尝试诱导抗病毒 抗伪狂犬病病毒的抗体（可能具有保护性）， 巨细胞病毒 表达主要和次要IgE单克隆抗体 将对独特型进行测序，以确定 这个IG类。 最后，敏感的定量技术， 携带独特型IgE抗苯胂酸抗体将用于启动 IgE调节的研究。 这些研究应该增加我们对抗体作用机制的认识 生物合成和调节。 关于结构变化的资料 抗体与我们对抗体生物合成的理解有关。 某些抗独特型抗体中抗原内部图像的研究 可能导致使用这种抗体作为疫苗或作为免疫调节剂， 涉及细胞表面受体的生理过程。 IgE研究 它的调节与控制直接反应有关， 过敏，包括过敏。

The research objectives are: (1) to obtain more information on the structure of immunoglobulins and on the nature of somatic variations that operate on germline genes. For antibodies of an idiotypic family, attempts will be made to correlate sequences with the 3-D structure of one or more members of the family; (2) to better define and quantify antiidiotypes that express an internal image of the original antigen, and to use the principle for induction of antiviral antibodies; (3) to study sequence variations among IgE antibodies of defined idiotype and to compare the nature and extent of these variations with those occurring in other classes; (4) to initiate studies on the regulation of IgE. Project 1 will be approached through X-ray crystallography, carried out by Dr. R. Poljak on monoclonal antibodies we are supplying, and by determination of amino acid sequences (inferred from mRNA sequences). The sequences of minor idiotypes, that are closely related to but divergent from a major cross-reactive idiotype, should add a dimension to the degree of variation on germline DNA sequences that can be investigated. We will quantify anti-Id expressing an internal image of the antigen in several systems and attempt to induce antiviral antibodies (possibly protective) against pseudorabies virus and cytomegalovirus. IgE monoclonal antibodies that express major and minor idiotypes will be sequenced to ascertain the patterns of variability in this Ig class. Finally, sensitive quantitative techniques for total and idiotype-bearing IgE antiphenylarsonate antibodies will be used to initiate studies of IgE regulation. These studies should add to our knowledge of mechanisms of antibody biosynthesis and regulation. Information on structural variations in antibodies is relevant to our understanding of antibody biosynthesis. Studies on internal images of antigens in certain antiidiotypic antibodies may lead to the use of such antibodies as vaccines or as modulators of physiological processes involving cell surface receptors. Studies of IgE and its regulation are relevant to the control of reactions of immediate hypersensitivity, including allergies.