Glucosidase inhibitors: new approaches to malting efficiency

葡萄糖苷酶抑制剂：提高麦芽效率的新方法

• 批准号: BB/I017291/1
• 负责人: Alison Smith
• 金额: $ 63.3万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FI017291%2F1
• 关键词: Glucosidase; inhibitors; new; approaches; malting

The brewing and distilling industries are of enormous economic importance to the UK. They have a major impact on farming because they use almost 2M tonnes of UK-grown barley (about one third of the crop, occupying one third of a million hectares of land) every year, they provide employment for tens of thousands of people, and their products are enjoyed not only in the UK but in many countries around the world. There is strong pressure on the industry to increase the efficiency with which barley grain is converted into beer and whisky. This is in part to maintain profitability, but also to reduce the production of waste and the amount of energy used in the conversion process. The basic conversion process occurs in four main stages. First, during malting, the barley grains are soaked in water then allowed to start to germinate. Inside the germinating grain, enzymes are produced that can convert the starch stored in the grain to sugars. Second, during kilning, the grain is heated to dry it out so that germination stops. Third, the grain is milled then mixed with hot water. During this mashing process, the enzymes convert the starch to sugars. Finally, the sugar-containing liquid is drained off and yeast is added. The yeast converts the sugars to alcohol. One of the major losses during the conversion of grain to beer and whisky occurs during malting. As soon as the enzymes are produced, they start to convert starch to sugars inside the seed, and the sugars fuel the growth of rootlets. Thus some of the starch store is lost before the mashing stage, reducing the potential yield of alcohol and resulting in the production of unwanted rootlets. This loss is between 5% and 10% of the starch. In the context of a market value of £20bn for the brewing industry alone, even a small reduction in the extent of starch loss during malting would have huge economic benefits. Because of the economic importance of this malting loss, several different methods to prevent rootlet growth have been tested. However these have not been applied commercially, because of cost, toxicity, or adverse effects on the quality of the malt. We have discovered that both rootlet growth and starch loss in germinating barley seeds can be reduced or prevented by the application of tiny amounts of natural plant products, called iminosugars. These products have the potential to reduce malting losses without undesirable side effects. Understanding how they work inside the seed will also provide new information that will help in developing better varieties of barley for brewing and distilling. In this project we will test natural products in a 'micromalting' system that mimics real malting, and identify which ones are suitable for commercial trials. We will use biochemical and molecular methods to discover precisely how these products prevent the growth of rootlets, and the loss of starch. This information will enable us to identify genes in barley that are important in determining the malting quality of the grain. To ensure that our research is relevant to the needs of the brewing and distilling industries, we will regularly consult an Advisory Panel that includes an expert on these industries, and also experts on barley grain germination, plant natural products, and malting.

酿酒和蒸馏行业对英国具有巨大的经济重要性。他们对农业有重大影响，因为他们每年使用近200万吨英国种植的大麦（约占作物的三分之一，占百万公顷土地的三分之一），他们为数万人提供就业机会，他们的产品不仅在英国，而且在世界各地的许多国家都很受欢迎。该行业面临着巨大的压力，要求提高大麦转化为啤酒和威士忌的效率。这在一定程度上是为了保持盈利能力，但也是为了减少废物的产生和转换过程中使用的能源。基本的转换过程分为四个主要阶段。首先，在制麦过程中，大麦粒被浸泡在水中，然后开始发芽。在发芽的谷物内部，产生了可以将谷物中储存的淀粉转化为糖的酶。第二，在烘干过程中，谷物被加热干燥，从而停止发芽。第三，谷物被磨碎，然后与热水混合。在糖化过程中，酶将淀粉转化为糖。最后，将含糖液体排出并加入酵母。酵母将糖转化为酒精。在谷物转化为啤酒和威士忌的过程中，主要的损失之一发生在制麦过程中。一旦这些酶产生，它们就开始在种子内将淀粉转化为糖，而糖则为小根的生长提供燃料。因此，在糖化阶段之前损失了一些淀粉储存，降低了酒精的潜在产量，并导致产生不需要的小根。这种损失在淀粉的5%至10%之间。在仅酿酒行业的市场价值就达到200亿英镑的背景下，即使在制麦过程中淀粉损失的程度略有减少，也会产生巨大的经济效益。由于这种发芽损失的经济重要性，已经测试了几种不同的方法来防止小根生长。然而，由于成本、毒性或对麦芽质量的不利影响，这些还没有在商业上应用。我们已经发现，通过应用少量的天然植物产品（称为亚氨基糖），可以减少或防止发芽大麦种子中的小根生长和淀粉损失。这些产品有可能减少麦芽损失，而没有不良的副作用。了解它们如何在种子内工作也将提供新的信息，这将有助于开发更好的大麦品种用于酿造和蒸馏。在这个项目中，我们将在一个模拟真实的麦芽制造的“微麦芽制造”系统中测试天然产品，并确定哪些产品适合商业试验。我们将使用生物化学和分子方法来精确地发现这些产品是如何防止小根的生长和淀粉的损失的。这些信息将使我们能够确定大麦中对决定谷物麦芽质量很重要的基因。为了确保我们的研究与酿造和蒸馏行业的需求相关，我们将定期咨询咨询咨询小组，其中包括这些行业的专家，以及大麦发芽，植物天然产品和麦芽制造方面的专家。

项目成果

The Maltase Involved in Starch Metabolism in Barley Endosperm Is Encoded by a Single Gene.

• DOI: 10.1371/journal.pone.0151642
• 发表时间: 2016
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Andriotis VM;Saalbach G;Waugh R;Field RA;Smith AM
• 通讯作者: Smith AM

Cell wall degradation is required for normal starch mobilisation in barley endosperm.

• DOI: 10.1038/srep33215
• 发表时间: 2016-09-13
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Andriotis VM;Rejzek M;Barclay E;Rugen MD;Field RA;Smith AM
• 通讯作者: Smith AM

High-Throughput In Vitro Screening for Inhibitors of Cereal a-Glucosidase.

谷物α-葡萄糖苷酶抑制剂的高通量体外筛选。

• DOI: 10.1007/978-1-4939-7874-8_9
• 发表时间: 2018
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Rugen MD