ALLERGIC REACTIVITY OF PARTHENIUM HYSTEROPORUS POLLEN

银耳花花粉的过敏反应性

• 批准号: 3134836
• 负责人: H JAMES WEDNER
• 金额: $ 10.36万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134836
• 关键词: Ambrosia; Compositae; India; United States; affinity chromatography; air sampling /monitoring; asthma; atopy; electrofocusing; enzyme linked immunosorbent assay; gel electrophoresis; human subject; hypersensitivity desensitization; immunologic assay /test; immunopathology diagnosis; monoclonal antibody; plant extracts; pollen; radioimmunoassay; respiratory hypersensitivity; rhinitis; seasons; serology /serodiagnosis; systematic biology; tissues

Parthenium hysterophorus (P.H.) is a member of the Asteraceae long thought to be non-allergenic. Recent evidence has indicated, however, that the pollen from this weed is airborne in large quantities and that a significant proportion of the atopic population in the United States Gulf coast from Texas to Florida (population more than 50 million) may be sensitized. To characterize the scope of the P.H. problem, plant surveys and pollen sampling will be performed, initially in Texas and later in other Gulf coast areas. Vouchered samples of P.H. pollen will be collected and aqueous pollen extracts will be used for RAST testing on sera obtained in Austin and Corpus Christi, Texas (where high levels of P.H. are anticipated) from Madison, Wisconsin (where no P.H. is present), St. Louis (where occasional sensitivity is possible) and two clinics in India where sensitivity to P.H. may be a major problem and where no ragweed (a potential cross-reacting pollen) is found. Cross-reactivity of P.H. with other members of the Asteraceae will be determined initially by RAST inhibition studies, and broader studies of cross-reactivity will be done using an ELISA assay. The allergenic proteins in the P.H. pollen extract will be identified by Western blot analysis using sera positive for P.H. by RAST or ELISA analysis. Polyclonal and monoclonal antibodies raised to the allergenic proteins will be used to purify each of the proteins in sufficient quantity for skin testing patients in the U.S. gulf coast and in India. The pattern of allergenic proteins in P.H. will be compared to that found in other Asteraceae to produce a P.H. sensitivity in individuals presenting with fall seasonal pollinosis will be performed. This study including RAST, skin testing and bronchial inhalation challenge will demonstrate the true prevalence of P.H. sensitivity and correlate RAST or skin sensitivity with end organ response.

银胶菊（Parthenium heterophorus（P.H.）是菊科的一员 长期以来被认为是非过敏性的。 最近的证据表明， 然而，这种杂草的花粉大量通过空气传播 数量和一个显着比例的特应性 从德克萨斯到佛罗里达的美国墨西哥湾沿岸的人口 （人口超过5000万）可能会受到影响。 到 描述P.H.的范围。问题，工厂调查和 花粉采样将进行，最初在得克萨斯州，后来在 其他海湾地区。 p.H.花粉会 收集的花粉和水提取物将用于RAST 对得克萨斯州奥斯汀和科珀斯克里斯蒂获得的血清进行检测 （高水平的pH值。预计）从麦迪逊， 威斯康星州（那里没有公共卫生部。圣路易斯（St. Louis）， 敏感性是可能的）和印度的两个诊所， P.H.可能是一个主要问题，如果没有豚草（潜在的 交叉反应花粉）。与 菊科的其他成员将首先由 RAST抑制研究和更广泛的交叉反应性研究 将使用ELISA测定法进行。 过敏原中的蛋白质 P.H.花粉提取物将通过蛋白质印迹分析来鉴定 使用对PH呈阳性的血清。通过RAST或ELISA分析。 针对过敏原的多克隆和单克隆抗体 蛋白质将被用来纯化每一种蛋白质， 美国墨西哥湾沿岸和 印度 P.H.进行比较 与其他菊科植物中发现的产生PH值。敏感性 出现秋季季节性花粉症的人将 执行。 本研究包括RAST、皮肤试验和支气管 吸入性激发试验将证明PH的真实患病率。 敏感性和相关RAST或皮肤敏感性与终末器官 反应