ALLERGIC REACTIVITY OF PARTHENIUM HYSTEROPORUS POLLEN

百里香(Parthum hysrophorus(P.H.)是菊科植物的一员 长期以来一直被认为是不会过敏的。最近的证据表明， 然而，这种杂草的花粉是大量通过空气传播的 数量和相当大比例的特应性 从德克萨斯州到佛罗里达州的美国墨西哥湾沿岸的人口 (人口超过5000万)可能会被敏感化。至 描述P.H.问题的范围、植物调查和 将进行花粉采样，最初是在德克萨斯州，后来是 其他墨西哥湾沿岸地区。有担保的P.H.花粉样本将被 采集的和含水的花粉提取物将用于Rast 对在德克萨斯州奥斯汀和科珀斯克里斯蒂获得的血清进行检测 (预计P.H.水平会很高)来自麦迪逊， 威斯康星州(那里没有P.H.)，圣路易斯(偶尔 敏感是可能的)和印度的两家诊所，那里对 P.H.可能是一个主要问题，在没有豚草(潜在的 交叉反应的花粉)。P.H.与Ph的交叉反应 菊科的其他成员将初步确定为 RAST抑制研究，以及更广泛的交叉反应研究 将使用ELISA法进行检测。人体内的致敏蛋白 用Western印迹分析鉴定P.H.花粉提取液 用RAST或ELISA法检测P.H.阳性的血清。 针对过敏原的多克隆和单克隆抗体 蛋白质将被用来纯化每一种足够的蛋白质 美国墨西哥湾沿岸和中国的皮试患者数量 印度。将比较P.H.中过敏蛋白的模式 与在其他菊科植物中发现的产生P.H.敏感性的 出现秋季季节性花粉症的个人将是 已执行。这项研究包括RAST、皮试和支气管 吸入性挑战将证明P.H.的真实患病率。 敏感度及其与末梢器官的关系 回应。

Parthenium hysterophorus (P.H.) is a member of the Asteraceae long thought to be non-allergenic. Recent evidence has indicated, however, that the pollen from this weed is airborne in large quantities and that a significant proportion of the atopic population in the United States Gulf coast from Texas to Florida (population more than 50 million) may be sensitized. To characterize the scope of the P.H. problem, plant surveys and pollen sampling will be performed, initially in Texas and later in other Gulf coast areas. Vouchered samples of P.H. pollen will be collected and aqueous pollen extracts will be used for RAST testing on sera obtained in Austin and Corpus Christi, Texas (where high levels of P.H. are anticipated) from Madison, Wisconsin (where no P.H. is present), St. Louis (where occasional sensitivity is possible) and two clinics in India where sensitivity to P.H. may be a major problem and where no ragweed (a potential cross-reacting pollen) is found. Cross-reactivity of P.H. with other members of the Asteraceae will be determined initially by RAST inhibition studies, and broader studies of cross-reactivity will be done using an ELISA assay. The allergenic proteins in the P.H. pollen extract will be identified by Western blot analysis using sera positive for P.H. by RAST or ELISA analysis. Polyclonal and monoclonal antibodies raised to the allergenic proteins will be used to purify each of the proteins in sufficient quantity for skin testing patients in the U.S. gulf coast and in India. The pattern of allergenic proteins in P.H. will be compared to that found in other Asteraceae to produce a P.H. sensitivity in individuals presenting with fall seasonal pollinosis will be performed. This study including RAST, skin testing and bronchial inhalation challenge will demonstrate the true prevalence of P.H. sensitivity and correlate RAST or skin sensitivity with end organ response.