ALLERGIC REACTIVITY OF PARTHENIUM HYSTEROPORUS POLLEN

银耳花花粉的过敏反应性

• 批准号: 3134838
• 负责人: H JAMES WEDNER
• 金额: $ 9.31万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134838
• 关键词: Ambrosia; Compositae; India; United States; affinity chromatography; air sampling /monitoring; asthma; atopy; electrofocusing; enzyme linked immunosorbent assay; gel electrophoresis; human subject; hypersensitivity desensitization; immunologic assay /test; immunopathology diagnosis; laboratory mouse; laboratory rabbit; laboratory rat; monoclonal antibody; plant extracts; plant proteins; pollen; radioimmunoassay; respiratory hypersensitivity; rhinitis; seasons; serology /serodiagnosis; systematic biology; tissues

Parthenium hysterophorus (P.H.) is a member of the Asteraceae long thought to be non-allergenic. Recent evidence has indicated, however, that the pollen from this weed is airborne in large quantities and that a significant proportion of the atopic population in the United States Gulf coast from Texas to Florida (population more than 50 million) may be sensitized. To characterize the scope of the P.H. problem, plant surveys and pollen sampling will be performed, initially in Texas and later in other Gulf coast areas. Vouchered samples of P.H. pollen will be collected and aqueous pollen extracts will be used for RAST testing on sera obtained in Austin and Corpus Christi, Texas (where high levels of P.H. are anticipated) from Madison, Wisconsin (where no P.H. is present), St. Louis (where occasional sensitivity is possible) and two clinics in India where sensitivity to P.H. may be a major problem and where no ragweed (a potential cross-reacting pollen) is found. Cross-reactivity of P.H. with other members of the Asteraceae will be determined initially by RAST inhibition studies, and broader studies of cross-reactivity will be done using an ELISA assay. The allergenic proteins in the P.H. pollen extract will be identified by Western blot analysis using sera positive for P.H. by RAST or ELISA analysis. Polyclonal and monoclonal antibodies raised to the allergenic proteins will be used to purify each of the proteins in sufficient quantity for skin testing patients in the U.S. gulf coast and in India. The pattern of allergenic proteins in P.H. will be compared to that found in other Asteraceae to produce a P.H. sensitivity in individuals presenting with fall seasonal pollinosis will be performed. This study including RAST, skin testing and bronchial inhalation challenge will demonstrate the true prevalence of P.H. sensitivity and correlate RAST or skin sensitivity with end organ response.

龙葵（Parthenium hysterophorus， P.H.）是菊科植物之一

项目成果

Grass aeropollen of the western United States Gulf Coast.

美国墨西哥湾西部海岸的草气花粉。

• DOI: 10.1159/000236168
• 发表时间: 1992
• 期刊: International archives of allergy and immunology
• 影响因子: 2.8
• 作者: Lewis,WH;Dixit,AB;Wedner,HJ
• 通讯作者: Wedner,HJ

Asteraceae aeropollen of the western United States Gulf Coast.

美国墨西哥湾西部海岸的菊科气花粉。

• 发表时间: 1991
• 期刊: Annals of allergy
• 作者: Lewis,WH;Dixit,AB;Wedner,HJ
• 通讯作者: Wedner,HJ