How to build a biofilm

如何构建生物膜

• 批准号: BB/I019464/1
• 负责人: Nicola Stanley-Wall
• 金额: $ 44.35万
• 依托单位: University of Dundee
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FI019464%2F1
• 关键词: How; build; biofilm

A house is made from basic components that include bricks, roof tiles and mortar. Each part is essential to allow the building to be constructed. In addition the component parts need to be assembled in the correct order, and with the correct spatial arrangement, for the house to be strong and secure. Following this analogy, biofilms are communities of microbes (where the cells can be considered the bricks and roof tiles) that are surrounded by an extracellular matrix (the mortar) that provides structure. When living in the biofilm the microbes benefit and become resistant to antibiotics and other antimicrobial agents. This can result in the formation of chronic antibiotic resistant infections. We are interested in how a biofilm is built. The main component parts that are needed have been identified and in this proposal we aim to understand how they are assembled. Understanding how they are assembled will allow the development of novel strategies to block assembly in the natural environment and therefore guide future development of novel antimicrobial compounds for chronic debilitating biofilm infections.

房子是由基本组成部分，包括砖，屋顶瓦片和砂浆。每一个部分都是必不可少的，以允许建筑物被建造。此外，组成部分需要以正确的顺序组装，并具有正确的空间布置，以使房屋坚固和安全。按照这个类比，生物膜是微生物的群落（其中细胞可以被认为是砖块和屋顶瓦片），它们被提供结构的细胞外基质（砂浆）包围。当生活在生物膜中时，微生物受益并对抗生素和其他抗菌剂产生耐药性。这可能导致慢性抗生素耐药性感染的形成。我们感兴趣的是生物膜是如何形成的。所需的主要组成部分已经确定，在本提案中，我们的目标是了解它们是如何组装的。了解它们是如何组装的将允许开发新的策略来阻止在自然环境中的组装，从而指导未来开发用于慢性衰弱性生物膜感染的新型抗微生物化合物。

项目成果

The Bacterial Hydrophobin BslA is a Switchable Ellipsoidal Janus Nanocolloid

• DOI: 10.1021/acs.langmuir.5b02347
• 发表时间: 2015-10-27
• 期刊: LANGMUIR
• 影响因子: 3.9
• 作者: Brandani, Giovanni B.;Schor, Marieke;Zachariae, Ulrich
• 通讯作者: Zachariae, Ulrich

Enzymes in action: an interactive activity designed to highlight positive attributes of extracellular enzymes synthesized by microbes.

• DOI: 10.1128/jmbe.v15i2.737
• 发表时间: 2014-12
• 期刊: Journal of microbiology & biology education
• 影响因子: 1.9
• 作者: Gillespie RM;Stanley-Wall NR
• 通讯作者: Stanley-Wall NR

Biofilm formation by Bacillus subtilis: new insights into regulatory strategies and assembly mechanisms.

• DOI: 10.1111/mmi.12697
• 发表时间: 2014-08
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Cairns LS;Hobley L;Stanley-Wall NR
• 通讯作者: Stanley-Wall NR

Giving structure to the biofilm matrix: an overview of individual strategies and emerging common themes.

• DOI: 10.1093/femsre/fuv015
• 发表时间: 2015-09
• 期刊: FEMS microbiology reviews
• 影响因子: 11.3
• 作者: Hobley L;Harkins C;MacPhee CE;Stanley-Wall NR
• 通讯作者: Stanley-Wall NR

Norspermidine is not a self-produced trigger for biofilm disassembly.

• DOI: 10.1016/j.cell.2014.01.012
• 发表时间: 2014-02-13
• 期刊: Cell
• 影响因子: 64.5
• 作者: Hobley L;Kim SH;Maezato Y;Wyllie S;Fairlamb AH;Stanley-Wall NR;Michael AJ
• 通讯作者: Michael AJ