Biology and physics at the biofilm surface

生物膜表面的生物学和物理学

• 批准号: BB/L006804/1
• 负责人: Nicola Stanley-Wall
• 金额: $ 46.49万
• 依托单位: University of Dundee
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL006804%2F1
• 关键词: Biology; physics; biofilm; surface

Biofilms are communities of microbes that are surrounded by an extracellular matrix. The matrix functions like a glue to provide structure and protection to the resident microbes. An everyday example of a biofilm is dental plaque. As exemplified by dental plaque, microbes benefit from living in a biofilm by gaining access to hard-to-source nutrients and becoming difficult to remove from a surface. In other environments or situations microbes living in biofilms become resistant to antibiotics and other antimicrobial agents resulting in chronic infections or blockages in pipes and catheters. We are interested in how the biofilm is assembled. The main component parts that are needed have been identified and in this proposal we aim to understand how one of them assembles to form a protective coat around the members of the biofilm community. Understanding this assembly process will allow the development of novel strategies to block, reverse, or enhance biofilm assembly in the natural environment and guide future developments of novel antimicrobial compounds for chronic debilitating biofilm infections.

生物膜是被细胞外基质包围的微生物群落。基质的功能就像胶水一样，为常驻微生物提供结构和保护。生物膜的日常例子是牙菌斑。如牙菌斑所示，微生物通过获得难以获得的营养物质并变得难以从表面去除而受益于生活在生物膜中。在其他环境或情况下，生活在生物膜中的微生物对抗生素和其他抗微生物剂产生耐药性，导致慢性感染或管道和导管堵塞。我们感兴趣的是生物膜是如何组装的。所需的主要组成部分已经确定，在本提案中，我们的目标是了解其中一个如何组装，以形成生物膜社区成员周围的保护涂层。了解这种组装过程将允许开发新的策略来阻断，逆转或增强自然环境中的生物膜组装，并指导未来开发用于慢性衰弱生物膜感染的新型抗微生物化合物。

项目成果

Biofilm formation by Bacillus subtilis: new insights into regulatory strategies and assembly mechanisms.

• DOI: 10.1111/mmi.12697
• 发表时间: 2014-08
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Cairns LS;Hobley L;Stanley-Wall NR
• 通讯作者: Stanley-Wall NR

Giving structure to the biofilm matrix: an overview of individual strategies and emerging common themes.

• DOI: 10.1093/femsre/fuv015
• 发表时间: 2015-09
• 期刊: FEMS microbiology reviews
• 影响因子: 11.3
• 作者: Hobley L;Harkins C;MacPhee CE;Stanley-Wall NR
• 通讯作者: Stanley-Wall NR

An alternate route to phosphorylating DegU of Bacillus subtilis using acetyl phosphate.

• DOI: 10.1186/s12866-015-0410-z
• 发表时间: 2015-03-31
• 期刊: BMC microbiology
• 影响因子: 4.2
• 作者: Cairns LS;Martyn JE;Bromley K;Stanley-Wall NR
• 通讯作者: Stanley-Wall NR

Interfacial self-assembly of a bacterial hydrophobin

• DOI: 10.1073/pnas.1419016112
• 发表时间: 2015-04-28
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Bromley, Keith M.;Morris, Ryan J.;MacPhee, Cait. E.
• 通讯作者: MacPhee, Cait. E.

Formation of functional, non-amyloidogenic fibres by recombinant Bacillus subtilis TasA.

• DOI: 10.1111/mmi.13985
• 发表时间: 2018-12
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Erskine E;Morris RJ;Schor M;Earl C;Gillespie RMC;Bromley KM;Sukhodub T;Clark L;Fyfe PK;Serpell LC;Stanley-Wall NR;MacPhee CE
• 通讯作者: MacPhee CE